FIGURE SUMMARY
Title

Targeted two-photon chemical apoptotic ablation of defined cell types in vivo

Authors
Hill, R.A., Damisah, E.C., Chen, F., Kwan, A.C., Grutzendler, J.
Source
Full text @ Nat. Commun.
Fig. 10

Ablation of neuromast hair cells in the zebrafish lateral line.

(a) Schematic depicting zebrafish neuromast hair cells of the lateral line labeled with H33342. (b) Low magnification live image of an anesthetized KDR-GFP transgenic zebrafish with the neuromasts labelled with H33342 (yellow arrows) and GFP-labelled vessels shown in green. (c) High-resolution live images of single neuromasts with hair cells labelled in the Prox1-RFP transgenic line and cell nuclei labelled with H33342. (d) Two hair cells targeted for 2Phatal (yellow arrows) and imaged before, immediately after and at 6 h showing disappearance of the targeted cells. Numbers designate single cells imaged over time. (e) Time-lapse sequence showing nuclear pyknosis, extrusion of the condensed nucleus and disappearance of the targeted cell (yellow arrows). (f) Traces showing the time course of the apoptotic progression for nine cells targeted for 2Phatal from three separate fish.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.
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