Rab5 plays a crucial role in apical extrusion of v-Src?expressing cells in the EVL of zebrafish embryos. (A) Confocal images of a Rab5-YFP?expressing zebrafish embryo (at 10?11 h postfertilization, 3 h after tamoxifen treatment) with RFP?v-Src in a mosaic manner. (Scale bar, 20 ?m.) (B and C) Effect of expression of Rab5DN on apical extrusion of v-Src?expressing cells. Embryos were injected with the GFP?v-Src?expressing vector with or without the Rab5DN cassette. Representative images are shown in B. Arrows indicate apically extruded v-Src?expressing cells. (Scale bar, 10 ?m.) (C) Quantification of apical extrusion of v-Src?expressing cells. Data are mean ± SD from four independent experiments. *P < 0.05; n = 490 and 364 cells.