FIGURE SUMMARY
Title

Engineering FKBP-Based Destabilizing Domains to Build Sophisticated Protein Regulation Systems

Authors
An, W., Jackson, R.E., Hunter, P., Gögel, S., van Diepen, M., Liu, K., Meyer, M.P., Eickholt, B.J.
Source
Full text @ PLoS One
Fig. 4

Engineered FKBP** permits regulation of PTEN function in the zebrafish.

(A) The migration and growth of stable FKBP**-PTEN expressing U87MG cell clones injected into zebrafish embryos can be inhibited by Shld1. Prior to microinjection into the perivitelline cavity of 2 days post fertilization (dpf) zebrafish embryos, cells were pre-stained with DiI. Embryos were then transferred into fish water with (n = 11) or without (n = 13) Shld1 (4 ?M) and maintained for 24h. Images were taken immediately after injections (0 days post injections, dpi) and after 24 h (1dpi). (B) The migration indices and the increase in tumor mass size of transplanted cells were quantified. ** p<0.01; ***p<0.001.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
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