PCAIs disrupt the formation of zebrafish intersegmental vessels (ISVs) and subintestinal veins (SIVs). Transgenic (fli1: EGFP) Zebrafish embryos, 24 hpf were treated with NSL-BA-040, NSL-BA-055 and NSL-100 for 48 h and imaged as described in the methods. (A) Zebrafish anatomic regions highlighting angiogenesis targets: intersegmental vessels (ISV), dorsal longitudinal anastomotic vessel (DLAV), dorsal aorta (DA), subintestinal vein (SIV) and duct of cuvier (DC). (B) Zebrafish ISVs plots of the means ± SEM, N = 6–8. Significance (**p < 0.01 and ***p < 0.001). (C) and (D) Representative images and intensity profiles of zebrafish ISV and SIV, respectively depicting the effect of the compounds. The peak analysis for each compound was fixed based on the intensity of its untreated control.