Hypo-enhancers show reproducible expression pattern in enhancer assays. (a,b) Genome browser views of the two hypo-enhancers used in panel c. ChIP-Seq data and DNA methylation data are displayed as indicated from 4hpf old embryos. Normalized ChIP-Seq enrichments and fractional methylation are indicated at the left site of the image. Blue box indicates the hypo-enhancer. The blue arrow indicates the orientation of the gene. Blue horizontal bars indicate HMRs. (c) in vivo enhancer assay of hypo-enhancers located within 20kb of the indicated genes (see genome browser view S2a,b). The images were taken 24hpf and 48hpf of tbx2a and dachA respectively. (d,e) Genome browser view of the two hypo-enhancers tested in f and g. ChIP-Seq data and DNA methylation data are displayed as indicated from 4hpf old embryos. Normalized ChIP-Seq enrichments and fractional methylation are indicated at the left site of the image. Blue box indicates the hypo-enhancer. The blue arrow indicates the orientation of the gene. Blue horizontal bars indicate HMRs. (f,g) in vivo enhancer assay of hypo-enhancers located within 20kb of the indicated genes. The images were taken 3dpf and 10hpf of gsc and unxc4.1, respectively.