Gene disruption by SaCas9. (A) Schematic representation of the CRISPR/SaCas9 system, including SaCas9 and the gRNA. SaCas9 flanked by two NLS signals is driven by SP6 promoter, and the gRNA is driven by T7 promoter. The PAM is highlighted in red. The target site is labeled by blue color. (B) Phenotype of embryos by targeting the tyr gene. Scale bar = 500 µm. (C) Phenotype of embryos by targeting the EGFP transgene. Scale bar = 500 µm. (D-E) Sequencing results at the tyr and EGFP targets. Target sequence (blue), PAM region (red), deletion (red dashes) and insertions (lower case letters in green) are indicated. The numbers of mutant alleles are indicated in [×] brackets.