- Title
-
Epidermal growth factor suppresses intestinal epithelial cell shedding via a MAPK dependent pathway
- Authors
- Miguel, J.C., Maxwell, A.A., Hsieh, J.J., Harnisch, L.C., Al Alam, D., Polk, D.B., Lien, C.L., Watson, A.J., Frey, M.R.
- Source
- Full text @ J. Cell Sci.
EGF inhibits cell shedding in zebrafish intestine via MAPK signaling. (A) Zebrafish midgut tissue was fixed and stained with Rhodamine–phalloidin (red, F-actin) and DAPI (blue, nuclei). Examples of (i) early, (ii) late and (iii) completion of shedding process shown. Arrow, example saccular stage event. (B) Fish were treated intraperitoneally with vehicle (Control), EGF or AG1478 for 4 h. Early-stage shedding funnels (arrows) per mm of tissue perimeter were counted (C). Scale bars: 50 µm. ***P<0.001 vs control, n=5 per condition. (D) Fish were treated intraperitoneally with vehicle (Control), EGF or U0126 for 4 h. Tissue was fixed, stained and shedding funnels counted; n=6–8 per condition as shown. Quantitative results are mean±s.e.m. ***P<0.001 (one-way ANOVA with Tukey's post-test analysis). |