FIGURE SUMMARY
Title

The homologous recombination component EEPD1 is required for genome stability in response to developmental stress of vertebrate embryogenesis

Authors
Chun, C., Wu, Y., Lee, S.H., Williamson, E.A., Reinert, B.L., Jaiswal, A.S., Nickoloff, J.A., Hromas, R.A.
Source
Full text @ Cell Cycle
Fig. 2

EEPD1 deletion causes developmental delay and death during Zebrafish embryogenesis. (A) Depletion of the HR nuclease EEPD1 by MO injection into zygotes results in delayed embryonic development or death. Averages ±SD for two determinations are plotted. A total of 115-240 embryos were scored per condition. *** indicates P < 0.0001 by Fisher exact test for the combined data from the two determinations. (B) Morphologic abnormalities in developing zebrafish depleted of EEPD1, especially in the somite region; representative embryos are shown at 2, 5, and 6 days post-fertilization (dpf).
Fig. 3

EEPD1 depletion prevents replication stress signaling. (A) Representative confocal immunofluorescent photomicrographs of the somite region of zebrafish embryos stained for γ-H2Ax. (B) Percentage of somite nuclei >5 γ-H2Ax foci. Values are averages (±SD) for 3-6 embryos per condition, 56–375 nuclei scored per embryo. *** indicates P < 0.0001, t-tests.

EXPRESSION / LABELING:
Antibody:
Fish:
Condition:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Prim-5
Fig. 4

EEPD1 depletion in zebrafish embryos causes genome instability with or exogenous replication stress induced by 4 hr treatment with 5 mM HU. (A) Representative photomicrographs of somite nuclei in zebrafish embryos stained with DAPI. Arrows indicate micronuclei. (B) Percentage of somite nuclei displaying micronuclei. Values are averages (±SD) for 6-16 embryos per condition, 115-421 nuclei scored per embryo. *** indicates P ≤ 0.0001, t-tests.

PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Cycle
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.