FIGURE 6. Inhibition activities of EPS273 on P. aeruginosa PAO1 infection to human A549 lung cells and zebrafish embryos. (A) EPS273 inhibits P. aeruginosa PAO1 infection to human A549 lung cells. The percent cell death was calculated using propidium iodide uptake into A549 lung cells after 8 h and normalized to cells in the absence of EPS273, of which the cell death was set as 100%. Error bars indicate the standard deviations of three measurements. ^∗P < 0.05 versus the control. (B) The cytotoxic analysis of EPS273 to A549 human lung cells. A549 human lung cells were treated with 0.1, 0.5, and 1 μg/mL of EPS273 in the absence of P. aeruginosa PAO1. The percent cell death was calculated using uptake into A549 lung cells after 8 h and normalized to lung cells treated with P. aeruginosa PAO1 in the absence of EPS273, of which the cell death was set as 100%. Error bars indicate the standard deviations of three measurements. ^∗P < 0.05 versus the control. (C) EPS273 inhibits P. aeruginosa PAO1 infection to zebrafish embryos. Zebrafish embryos were infected with P. aeruginosa PAO1 in the presence or absence of EPS273 and imaged after 24 h infection. All the pictures were taken with inverted microscope (Nikon TS100, Japan) at 40X.