- Title
-
Potential probiotic yeasts isolated from the fish gut protect zebrafish (Danio rerio) from a Vibrio anguillarum challenge
- Authors
- Caruffo, M., Navarrete, N., Salgado, O., Díaz, A., López, P., García, K., Feijóo, C.G., Navarrete, P.
- Source
- Full text @ Front Microbiol
Stereoscopic observation of the 5-([4,6-Dichlorotriazin-2-yl] amino) fluorescein hydrochloride (DTAF)-labeled V. anguillarum in zebrafish larvae. (A) 5 dpf wild type (WT) larvae were inoculated by immersion with 107 UFC/ml DTAF stained V. anguillarum: (i) lateral view scheme of the gut; (ii) lateral view of challenged larvae. (B) 5 dpf Tg(Lyz:DsRed)nz50 larvae inoculated by immersion with 107 UFC/ml DTAF-stained V. anguillarum (challenge) or sterile E3 as a control (control). (C) Quantification of neutrophils migration was performed in the selected area (yellow rectangle). |
Colonization studies and microscopic observation of the yeast–zebrafish interactions. (A) Microscopic observation at 0 and 5 dpi of larvae (n = 60) inoculated by immersion with 5 × 106 UFC/ml DTAF-fluorescent yeast. (B) Viable yeast count (UFC/larvae) of larvae inoculated by immersion with 5 × 106 UFC/ml DTAF-fluorescent yeast. |