Activity of the zlamin A promoter in transgenic zebrafish embryos at 14 hpf of development. (A) Tissue-specific expression of GFP driven by the 2.99 kb promoter region in Kupffer′s vesicle (K), boundary between the embryonic shield and blastodisc (B), and brain primordium (Bp). (B) Tissue-specific GFP expression driven by the 1.67 kb promoter segment. Similar patterns were obtained in two independent transgenic lines for each promoter fragment. CLSM images are shown for the lateral view, and regular fluorescence images for the dorsal and ventral views. Bar, 0.25 mm.

Activity of the zlamin A promoter in transgenic zebrafish embryos at 1–3 dpf of development. (A) Tissue-specific expression of GFP driven by the 2.99 kb promoter region in notochord (N), myotome (M), forebrain (F), otic vesicle (O), branchial arches (A), eye (E) and pectoral fin bud (PF). (B) Tissue-specific GFP expression driven by the 1.67 kb promoter segment. Similar patterns were obtained in two independent transgenic lines for each promoter fragment. CLSM images are shown. Bar, 0.25 mm.

Activity of zlamin A promoter fragments in zebrafish embryos in transient assays. Tissue-specific GFP expression driven by 1.67, 1.52, 1.41 and 0.91 kb promoter fragments in myotome (M), heart (H), notochord (N), head region (He) and epidermis (E) at 2.5 dpf. Bar, 1 mm.

Analysis of 86 bp putative muscle regulatory element. (A) Sequence of  1158/ 1243 bp indicating consensus binding sites for various transcription factors. (B) Luciferase reporter assays in C2C12 myoblasts. (C) GFP expression driven by 1.24 and 0.91 kb fragments in myotome (M), epidermis (E), heart (H) and pericardium (P) in 2.5 dpf embryos. (D) Luciferase reporter assays of 1.67 kb promoter segment with single, double or triple mutations in E-boxes E5, E6 and E7. ** p < 0.01.