- Title
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Endocytic trafficking of rac is required for the spatial restriction of signaling in cell migration
- Authors
- Palamidessi, A., Frittoli, E., Garré, M., Faretta, M., Mione, M., Testa, I., Diaspro, A., Lanzetti, L., Scita, G., and Di Fiore, P.P.
- Source
- Full text @ Cell
Expression of Rab5 Prevents PGCs from Reaching Their Target during Zebrafish Development and Alters the Migratory Shape of PGCs (A) PGCs, visualized through the expression of GFP:nos1-3′UTR and used as control, correctly migrated to the gonadal area (boxed) by 32 hpf (hours post fertilization). In all control embryos (188/188), PGCs reached the appropriate final gonadal target. (B and C) In more than 70% of embryos injected with Rab5a:nos1-3′UTR (200/280), a significant number of PGCs were found in ectopic positions throughout the body. Scale bars, 50 μm. (D) Still images from time-lapse movies showing PGCs coinjected with GFP:nos1-3′UTR and either empty vector (left, GFP, see also Movie S13) or RAB5a:nos1-3′UTR (middle/right, GFP+RAB5, see also Movie S13). Arrowheads indicate the extension of elongated protrusions, which were detected in 49/77 of Rab5-coinjected and 15/88 of control cells. Scale bars, 4 μm. |
Reprinted from Cell, 134(1), Palamidessi, A., Frittoli, E., Garré, M., Faretta, M., Mione, M., Testa, I., Diaspro, A., Lanzetti, L., Scita, G., and Di Fiore, P.P., Endocytic trafficking of rac is required for the spatial restriction of signaling in cell migration, 135-147, Copyright (2008) with permission from Elsevier. Full text @ Cell