ZFIN ID: ZDB-LAB-970908-15
Dawid, Igor B.
||Dawid, Igor B.
||Laboratory of Molecular Genetics
Program in Genomics of Differentiation
Eunice Kennedy Shriver National Institute of Child Health and Human Development
National Institutes of Health
Building 6B, Room 422
STATEMENT OF RESEARCH INTERESTS
GENOMIC FEATURES ORIGINATING FROM THIS LAB
all 14 genomic features
The molecular basis of vertebrate embryogenesis in the zebrafish Danio rerio and the amphibian Xenopus laevis is the focus of the research in this group. As a broad approach to this question a screen for genes expressed in differential patterns during embryogenesis has been carried out in the zebrafish. Over 300 of these clones were considered suitable for further study by partial sequence analysis and radiation hybrid mapping. Information on these genes is available on ZFIN and the laboratory web site. To help develop tools for molecular-genetic analysis of zebrafish development this laboratory collaborated with others on the establishment and refinement of a radiation hybrid map for this organism. A mapping tool is available on the laboratory web site.
ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Mutants affecting the development of the brain are being studied, showing in one example that ribosome biogenesis is essential for this process during the first 24 hr of embryogenesis. The role of several genes in early development has been studied. These include nodal-related factors, the vega1, 2 (vent, vox) homeobox genes, and gadd45 that is involved in somitogenesis. The Nma protein has been shown to be a feedback inhibitor of BMP signaling, while Wif1 is a Wnt binding protein that can inhibit the activity of several Wnt proteins. Much attention has been focused on factors modulating FGF signaling; Sef, a novel protein, and Mkp3, a MAP kinase phosphatase, were shown to modulate Fgf signaling in the early embryo. A long-standing interest in this laboratory concerns the role of the LIM-homeobox gene Lim1 which is being studied in Xenopus. Lim1 function requires interaction with a binding molecule, named Ldb, whose properties have also been studied. Lim1 is expressed in the organizer where it has a major role in controlling gastrulation movements through its activation of the adhesion molecule PAPC.
The application of DNA microarray technology is being explored in both the Xenopus and zebrafish systems. RNA expression profiles in explants dissected from the embryo and after manipulation of different signaling pathways are being generated.
Organisms: Xenopus laevis (African clawed frog), Danio rerio (zebrafish).
Genes of Interest: Xlim-1 (lim1, Lhx-1), Ldb, nodal related factors (cyclops), vega1 (vox), vega2 (vent), Wif1, nma, iroquis group genes, sef, mkp3, bap28.
Heffer, A., Marquart, G.D., Aquilina-Beck, A., Saleem, N., Burgess, H.A., Dawid, I.B. (2017) Generation and characterization of Kctd15 mutations in zebrafish. PLoS One. 12:e0189162
Kim, K.I., Jeong, H.B., Ro, H., Lee, J.H., Kim, C.D., Yoon, T.J. (2017) Inhibitory effect of 5-iodotubercidin on pigmentation. Biochemical and Biophysical Research Communications. 490(4):1282-1286
Yoo, K.W., Maddirevula, S., Kumar, A., Ro, H., Huh, T.L., Rhee, M. (2017) Sinup is essential for the integrity of centrosomes and mitotic spindles in zebrafish embryos. Animal cells and systems. 21:93-99
Kim, M.K., Bang, C.Y., Kim, M.Y., Lee, J.H., Ro, H., Choi, M.S., Kim, D.I., Jang, Y.P., Choung, S.Y. (2016) Traditional herbal prescription LASAP-C inhibits melanin synthesis in B16F10 melanoma cells and zebrafish. BMC complementary and alternative medicine. 16:223
Won, M., Lee, S., Choi, S., Ro, H., Kim, K.J., Kim, J.H., Kim, K.E., Kim, K.K. (2016) Identification and characterization of the RNA-binding protein Rbfox3 in zebrafish embryo. Biochemical and Biophysical Research Communications. 472(2):373-8
Toyama, R., Kim, M.H., Rebbert, M.L., Gonzales, J., Burgess, H., and Dawid, I.B. (2013) Habenular commissure formation in zebrafish is regulated by the pineal gland-specific gene unc119c. Developmental dynamics : an official publication of the American Association of Anatomists. 242(9):1033-42
Coffey, C.M., Solleveld, P.A., Fang, J., Roberts, A.K., Hong, S.K., Dawid, I.B., Laverriere, C.E., and Glasgow, E. (2013) Novel oxytocin gene expression in the hindbrain is induced by alcohol exposure: transgenic zebrafish enable visualization of sensitive neurons. PLoS One. 8(1):e53991
Park, H., Song, K.H., Jung, P.M., Kim, J.E., Ro, H., Kim, M.Y., and Ma, J.Y. (2013) Inhibitory Effect of Arctigenin from Fructus Arctii Extract on Melanin Synthesis via Repression of Tyrosinase Expression. Evidence-based complementary and alternative medicine : eCAM. 2013:965312
Takesono, A., Moger, J., Faroq, S., Cartwright, E., Dawid, I.B., Wilson, S.W., and Kudoh, T. (2012) Solute carrier family 3 member 2 (Slc3a2) controls yolk syncytial layer (YSL) formation by regulating microtubule networks in the zebrafish embryo. Proceedings of the National Academy of Sciences of the United States of America. 109(9):3371-3376
Dente, L., Gestri, G., Tsang, M., Kudoh, T., Wilson, S.W., Dawid, I.B., and Andreazzoli, M. (2011) Cloning and developmental expression of zebrafish pdzrn3. The International journal of developmental biology. 55(10-12):989-993
Znosko, W.A., Yu, S., Thomas, K., Molina, G.A., Li, C., Tsang, W., Dawid, I.B., Moon, A.M., and Tsang, M. (2010) Overlapping functions of Pea3 ETS transcription factors in FGF signaling during zebrafish development. Developmental Biology. 342(1):11-25
Cruz, C., Maegawa, S., Weinberg, E.S., Wilson, S.W., Dawid, I.B., and Kudoh, T. (2010) Induction and patterning of trunk and tail neural ectoderm by the homeobox gene eve1 in zebrafish embryos. Proceedings of the National Academy of Sciences of the United States of America. 107(8):3564-3569
Swanhart, L.M., Takahashi, N., Jackson, R.L., Gibson, G.A., Watkins, S.C., Dawid, I.B., and Hukriede, N.A. (2010) Characterization of an lhx1a transgenic reporter in zebrafish. The International journal of developmental biology. 54(4):731-736
Toyama, R., Chen, X., Jhawar, N., Aamar, E., Epstein, J., Reany, N., Alon, S., Gothilf, Y., Klein, D.C., and Dawid, I.B. (2009) Transcriptome analysis of the zebrafish pineal gland. Developmental dynamics : an official publication of the American Association of Anatomists. 238(7):1813-1826
Brown, J.D., Dutta, S., Bharti, K., Bonner, R.F., Munson, P.J., Dawid, I.B., Akhtar, A.L., Onojafe, I.F., Alur, R.P., Gross, J.M., Hejtmancik, J.F., Jiao, X., Chan, W.Y., and Brooks, B.P. (2009) Expression profiling during ocular development identifies 2 Nlz genes with a critical role in optic fissure closure. Proceedings of the National Academy of Sciences of the United States of America. 106(5):1462-1467
Pugacheva, E.M., Kwon, Y.W., Hukriede, N.A., Pack, S., Flanagan, P.T., Ahn, J.C., Park, J.A., Choi, K.S., Kim, K.W., Loukinov, D., Dawid, I.B., and Lobanenkov, V.V. (2006) Cloning and characterization of zebrafish CTCF: Developmental expression patterns, regulation of the promoter region, and evolutionary aspects of gene organization. Gene. 375:26-36
Hong, S.K., Haldin, C.E., Lawson, N.D., Weinstein, B.M., Dawid, I.B., and Hukriede, N.A. (2005) The zebrafish kohtalo/trap230 gene is required for the development of the brain, neural crest, and pronephric kidney. Proceedings of the National Academy of Sciences of the United States of America. 102(51):18473-18478
Kudoh, T., Concha, M.L., Houart, C., Dawid, I.B., and Wilson, S.W. (2004) Combinatorial Fgf and Bmp signalling patterns the gastrula ectoderm into prospective neural and epidermal domains. Development (Cambridge, England). 131(15):3581-3592
Tsang, M., Maegawa, S., Kiang, A., Habas, R., Weinberg, E., and Dawid, I.B. (2004) A role for MKP3 in axial patterning of the zebrafish embryo. Development (Cambridge, England). 131(12):2769-2779
Appelbaum, L., Toyama, R., Dawid, I.B., Klein, D.C., Baler, R., and Gothilf, Y. (2004) Zebrafish serotonin-N-acetyltransferase-2 gene regulation: Pineal-restrictive downstream module (PRDM) contains a functional E-box and three photoreceptor conserved elements. Molecular endocrinology (Baltimore, Md.). 18(5):1210-1221
Preger, E., Ziv, I., Shabtay, A., Sher, I., Tsang, M., Dawid, I.B., Altuvia, Y., and Ron, D. (2004) Alternative splicing generates an isoform of the human Sef gene with altered subcellular localization and specificity. Proceedings of the National Academy of Sciences of the United States of America. 101(5):1229-1234
Piotrowski, T., Ahn, D.-G., Schilling, T.F., Nair, S., Ruvinsky, I., Geisler, R., Rauch, G.-J., Haffter, P., Zon, L.I., Zhou, Y., Foott, H., Dawid, I.B., and Ho, R.K. (2003) The zebrafish van gogh mutation disrupts tbx1, which is involved in the DiGeorge deletion syndrome in humans. Development (Cambridge, England). 130(20):5043-5052
Watanabe, M., Rebbert, M.L., Andreazzoli, M., Takahashi, N., Toyama, R., Zimmerman, S., Whitman, M., and Dawid, I.B. (2002) Regulation of the Lim-1 gene is mediated through conserved FAST-1/FoxH1 sites in the first intron. Developmental dynamics : an official publication of the American Association of Anatomists. 225(4):448-456
Gothilf, Y., Toyama, R., Coon, S.L., Du, S.-J., Dawid, I.B., and Klein, D.C. (2002) Pineal-specific expression of green fluorescent protein under the control of the serotonin-N-acetyltransferase gene regulatory regions in transgenic zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 225(3):241-249
Hong, S.K., Kim, C.-H., Yoo, K.-W., Kim, H.S., Kudoh, T., Dawid, I.B., and Huh, T.L. (2002) Isolation and expression of a novel neuron-specific onecut homeobox gene in zebrafish. Mechanisms of Development. 112(1-2):199-202
Miyamoto, T., Kawahara, A., Teufel, A., Mukhopadhyay, M., Zhao, Y., Dawid, I.B., and Westphal, H. (2002) Mbx, a novel mouse homeobox gene. Development genes and evolution. 212(2):104-106
Hukriede, N., Fisher, D., Epstein, J., Joly, L., Tellis, P., Zhou, Y., Barbazuk, B., Cox, K., Fenton-Noriega, L., Hersey, C., Miles, J., Sheng, X., Song, A., Waterman, R., Johnson, S.L., Dawid, I.B., Chevrette, M., Zon, L.I., McPherson, J., and Ekker, M. (2001) The LN54 radiation hybrid map of zebrafish expressed sequences. Genome research. 11(12):2127-2132
Kudoh, T., Tsang, M., Hukriede, N.A., Chen, X., Dedekian, M., Clarke, C.J., Kiang, A., Schultz, S., Epstein, J.A., Toyama, R., and Dawid, I.B. (2001) A gene expression screen in zebrafish embryogenesis. Genome research. 11(12):1979-1987
Kudoh, T., Tsang, M., Hukriede, N.A., Chen, X., Dedekian, M., Clarke, C.J., Kiang, A., Schultz, S., Epstein, J.A., Toyama, R., and Dawid, I.B. (2001) A gene expression screen in zebrafish embryogenesis. ZFIN Direct Data Submission.
Tsang, M., Kim, R., De Caestecker, M.P., Kudoh, T., Roberts, A.B., and Dawid, I.B. (2000) Zebrafish nma is involved in TGFb family signaling. Genesis (New York, N.Y. : 2000). 28(2):47-57
Hukriede N,A., Joly, L., Tsang, M., Miles, J., Tellis, P., Epstein, J.A., Barbazuk, W.B., Li, F.N., Paw, B., Postlethwait, J.H., Hudson, T.J., Zon, L.I., McPherson, J.D., Chevrette, M., Dawid, I.B., Johnson, S.L., and Ekker, M. (1999) Radiation hybrid mapping of the zebrafish genome. Proceedings of the National Academy of Sciences of the United States of America. 96(17):9745-9750
Hsieh, J.C., Kodjabachian, L., Rebbert, M.L., Rattner, A., Smallwood, P.M., Samos, C.H., Nusse, R., Dawid, I.B., and Nathans, J. (1999) A new secreted protein that binds to Wnt proteins and inhibits their activities. Nature. 398(6726):431-436
Kobayashi, M., Toyama, R., Takeda, H., Dawid, I.B., and Kawakami, K. (1998) Overexpression of the forebrain-specific homeobox gene six3 induces rostral forebrain enlargement in zebrafish. Development (Cambridge, England). 125:2973-2982
Minucci, S., Leid, M., Toyama, R., Saint-Jeannet, J.P., Peterson, V.J., Horn, V., Ishmael, J.E., Bhattacharyya, N., Dey, A., Dawid, I.B., and Ozato, K. (1997) Retinoid X receptor (RXR) within the RXR-retinoic acid receptor heterodimer binds its ligand and enhances retinoid-dependent gene expression. Molecular and cellular biology. 17(2):644-655
Sheng, H.Z., Bertuzzi, S., Chiang, C., Shawlot, W., Taira, M., Dawid, I., and Westphal, H. (1997) Expression of murine Lhx5 suggests a role in specifying the forebrain. Developmental dynamics : an official publication of the American Association of Anatomists. 208(2):266-277
Minucci, S., Saint-Jeannet, J.P., Toyama, R., Scita, G., DeLuca, L.M., Taira, M., Levin, A.A., Ozato, K., and Dawid, I.B. (1996) Retinoid X receptor-selective ligands produce malformations in Xenopus embryos. Proceedings of the National Academy of Sciences of the United States of America. 93:1803-1807
Appel, B., Korzh, V., Glasgow, E., Thor, S., Edlund, T., Dawid, I.B., and Eisen, J.S. (1995) Motoneuron fate specification revealed by patterned LIM homeobox gene expression in embryonic zebrafish. Development (Cambridge, England). 121:4117-4125
Toyama, R., Curtiss, P.E., Otani, H., Kimura, M., Dawid, I.B., and Taira, M. (1995) The LIM class homeobox gene lim5: implied role in CNS patterning in Xenopus and zebrafish. Developmental Biology. 170:583-593
Toyama, R., O'Connell, M.L., Wright, C.V.E., Kuehn, M.R., and Dawid, I.B. (1995) Nodal induces ectopic goosecoid and lim1 expression and axis duplication in zebrafish. Development (Cambridge, England). 121:383-391