ZFIN ID: ZDB-LAB-970409-69
Reugels / Campos-Ortega Lab
Contact Person: Reugels, Alexander
Email: alexander.reugels@uni-koeln.de
URL: http://www.uni-koeln.de/math-nat-fak/ebio/
Address: Institut für Entwicklungsbiologie Universität zu Köln Gyrhofstrasse 17 D-50923 Köln GERMANY
Country: Germany
Phone: +49(0)221-4702618
Fax: +49(0)221-4705164
Line Designation: kca

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Proper spatial and temporal specification of cells during development is crucial for the generation of cellular diversity in the nervous system of complex organisms. We are interested in the mechanisms underlying the establishment of cellular polarity and the generation of neuronal cell lineages during neurulation in Danio rerio. We were able to show that neurulation in zebrafish embryos is characterised by oriented cell divisions and the progressive establishment of cellular polarity. Mitoses in the neural plate and neural tube are planar, but in the neural keel/rod stage the mitotic spindle rotates by 90°, causing cell divisions to occur perpendicular to the plane of the neuroepithelium. However, the mechanisms and molecules that establish cellular polarity and cause the stereotypic orientation of the mitotic spindle during neurulation are still largely unknown. In order to address this topic, we are currently analyzing the putative cell fate determinant Numb and the role of the Par-3/Par-6/aPKC complex in setting up apicobasal polarity and specifying the orientation of the mitotic spindle. A major aspect of our work is the in vivo time-lapse imaging of GFP-tagged fusion proteins via confocal laser scanning microscopy.

Boggetti, Barbara Post-Doc Reugels, Alexander Post-Doc von Trotha, Jakob Graduate Student
Riedl, Iris Research Staff Schenkel, Christel Research Staff Wagner, Thomas Research Staff

Lindemann, N., Kalix, L., Possiel, J., Stasch, R., Kusian, T., Köster, R.W., von Trotha, J.W. (2022) A comparative analysis of Danionella cerebrum and zebrafish (Danio rerio) larval locomotor activity in a light-dark test. Frontiers in behavioral neuroscience. 16:885775
Mattern, K., von Trotha, J.W., Erfle, P., Köster, R.W., Dietzel, A. (2020) NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish. Communications biology. 3:311
Löhr, H., Hess, S., Pereira, M.M.A., Reinoß, P., Leibold, S., Schenkel, C., Wunderlich, C.M., Kloppenburg, P., Brüning, J.C., Hammerschmidt, M. (2018) Diet-Induced Growth Is Regulated via Acquired Leptin Resistance and Engages a Pomc-Somatostatin-Growth Hormone Circuit. Cell Reports. 23:1728-1741
von Trotha, J.W., Vernier, P., Bally-Cuif, L. (2014) Emotions and motivated behavior converge on an amygdala-like structure in the zebrafish. The European journal of neuroscience. 40(9):3302-3315
Boggetti, B., Jasik, J., Takamiya, M., Strähle, U., Reugels, A.M., and Campos-Ortega, J.A. (2012) NBP, a zebrafish homolog of human Kank3, is a novel Numb interactor essential for epidermal integrity and neurulation. Developmental Biology. 365(1):164-174
Alexandre, P., Reugels, A.M., Barker, D., Blanc, E., and Clarke, J.D. (2010) Neurons derive from the more apical daughter in asymmetric divisions in the zebrafish neural tube. Nature Neuroscience. 13(6):673-679
Tawk, M., Araya, C., Lyons, D.A., Reugels, A.M., Girdler, G.C., Bayley, P.R., Hyde, D.R., Tada, M., and Clarke, J.D. (2007) A mirror-symmetric cell division that orchestrates neuroepithelial morphogenesis. Nature. 446(7137):797-800
Reugels, A.M., Boggetti, B., Scheer, N., and Campos-Ortega, J.A. (2006) Asymmetric localization of Numb:EGFP in dividing neuroepithelial cells during neurulation in Danio rerio. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(4):934-948
von Trotha, J.W., Campos-Ortega, J.A., and Reugels, A.M. (2006) Apical localization of ASIP/PAR-3:EGFP in zebrafish neuroepithelial cells involves the oligomerization domain CR1, the PDZ domains, and the C-terminal portion of the protein. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(4):967-977
Hans, S., Scheer, N., Riedl, I., Von Weizsacker, E., Blader, P., and Campos-Ortega, J.A. (2004) her3, a zebrafish member of the hairy-E(spl) family, is repressed by Notch signalling. Development (Cambridge, England). 131(12):2957-2969
Schlombs, K., Wagner, T., and Scheel, J. (2003) Site-1 protease is required for cartilage development in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 100(24):14024-14029
Geldmacher-Voss, B., Reugels, A.M., Pauls, S., and Campos-Ortega, J.A. (2003) A 90° rotation of the mitotic spindle changes the orientation of mitoses of zebrafish neuroepithelial cells. Development (Cambridge, England). 130(16):3767-3780
Scheer, N., Riedl, I., Warren, J.T., Kuwada, J.Y., and Campos-Ortega, J.A. (2002) A quantitative analysis of the kinetics of Gal4 activator and effector gene expression in the zebrafish. Mechanisms of Development. 112(1-2):9-14
Boggetti, B., Argenton, F., Haffter, P., Bianchi, M.E., Cotelli, F., and Beltrame, M. (2000) Cloning and expression pattern of a zebrafish homolog of forkhead activin signal transducer (FAST), a transcription factor mediating Nodal-related signals. Mechanisms of Development. 99(1-2):187-190