ZFIN ID: ZDB-LAB-180207-3
Qing Deng Lab
PI/Director: Deng, Qing
Contact Person: Deng, Qing
Email: qingdeng@purdue.edu
URL: https://www.bio.purdue.edu/lab/deng/
Address: Department of Biological Sciences Purdue University LILY G201 915 W. State Street West Lafayette, IN 47907
Country: United States
Phone: 765-496-3237
Fax: 765-494-0876
Line Designation: pu

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Our lab is interested in the mechanisms that regulate neutrophil migration and activation, more specifically their response to tissue injury and infection. The neutrophil is a hallmark of inflammation and neutrophilic inflammation is the driving force behind a spectrum of human inflammatory diseases. Restraining neutrophilic inflammation will help alleviate diseases such as arthritis, cancer and auto-inflammation. We are also interested in understanding pathogen-host interactions using the zebrafish model and the strength relies on the combination of the genetics in pathogens and the host. We are creating a lab where happy scientists do solid science.

One of our current projects is to characterize the role of miRNAs in regulating neutrophil functions. Using next generation sequencing, we have identified miRNAs that are enriched in neutrophils. The focus to characterize the contribution of each individual miRNA in regulating neutrophil motility and response to tissue injury or infection using a combination of genetics and imaging tools.

Jeffries, Jacob Graduate Student Kim, Daniel Graduate Student Syahirah Mohd Sabri, Ramizah Graduate Student
Wang, Tianqi Graduate Student Wang, Yueyang Graduate Student

Chang, Y., Syahirah, R., Oprescu, S.N., Wang, X., Jung, J., Cooper, S.H., Torregrosa-Allen, S., Elzey, B.D., Hsu, A.Y., Randolph, L.N., Sun, Y., Kuang, S., Broxmeyer, H.E., Deng, Q., Lian, X., Bao, X. (2022) Chemically-defined generation of human hemogenic endothelium and definitive hematopoietic progenitor cells. Biomaterials. 285:121569
Hsu, A.Y., Wang, T., Syahirah, R., Liu, S., Li, K., Zhang, W., Wang, J., Cao, Z., Tian, S., Matosevic, S., Staiger, C.J., Wan, J., Deng, Q. (2022) Rora Regulates Neutrophil Migration and Activation in Zebrafish. Frontiers in immunology. 13:756034
Wang, Y., Hsu, A.Y., Walton, E.M., Park, S.J., Syahirah, R., Wang, T., Zhou, W., Ding, C., Lemke, A.P., Zhang, G., Tobin, D.M., Deng, Q. (2021) A robust and flexible CRISPR/Cas9 based system for neutrophil-specific gene inactivation in zebrafish. Journal of Cell Science. 134(8):
Zhou, W., Hsu, A.Y., Wang, Y., Syahirah, R., Wang, T., Jeffries, J., Wang, X., Mohammad, H., Seleem, M.N., Umulis, D., Deng, Q. (2020) Mitofusin 2 regulates neutrophil adhesive migration and the actin cytoskeleton. Journal of Cell Science. 133(17):
Cash, J.N., Chandan, N.R., Hsu, A.Y., Sharma, P.V., Deng, Q., Smrcka, A.V., Tesmer, J.J.G. (2020) Discovery of small molecules that target the P-Rex1 PIP3-binding site and inhibit P-Rex1-dependent functions in neutrophils. Molecular pharmacology. 97(3):226-236
Hsu, A.Y., Wang, D., Liu, S., Lu, J., Syahirah, R., Bennin, D.A., Huttenlocher, A., Umulis, D.M., Wan, J., Deng, Q. (2019) Phenotypical microRNA screen reveals a noncanonical role of CDK2 in regulating neutrophil migration. Proceedings of the National Academy of Sciences of the United States of America. 116(37):18561-18570
Hsu, A.Y., Liu, S., Syahirah, R., Brasseale, K.A., Wan, J., Deng, Q. (2019) Inducible overexpression of zebrafish microRNA-722 suppresses chemotaxis of human neutrophil like cells. Molecular immunology. 112:206-214
Weaver, C.J., Terzi, A., Roeder, H., Gurol, T., Deng, Q., Fai Leung, Y., Suter, D.M. (2018) nox2/cybb deficiency affects zebrafish retinotectal connectivity. The Journal of neuroscience : the official journal of the Society for Neuroscience. 38(26):5854-5871
Hsu, A.Y., Gurol, T., Sobreira, T.J.P., Zhang, S., Moore, N., Cai, C., Zhang, Z.Y., Deng, Q. (2018) Development and Characterization of an Endotoxemia Model in Zebra Fish. Frontiers in immunology. 9:607
Zhou, W., Cao, L., Jeffries, J., Zhu, X., Staiger, C.J., Deng, Q. (2018) Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish. Disease models & mechanisms. 11(3)
Zhou, W., Pal, A.S., Hsu, A.Y., Gurol, T., Zhu, X., Wirbisky-Hershberger, S.E., Freeman, J.L., Kasinski, A.L., Deng, Q. (2018) MicroRNA-223 Suppresses the Canonical NF-κB Pathway in Basal Keratinocytes to Dampen Neutrophilic Inflammation. Cell Reports. 22:1810-1823
Hsu, A.Y., Wang, D., Gurol, T., Zhou, W., Zhu, X., Lu, H.Y., Deng, Q. (2017) Overexpression of microRNA-722 fine-tunes neutrophilic inflammation by inhibiting Rac2 in zebrafish.. Disease models & mechanisms. 10(11):1323-1332
Powell, D., Tauzin, S., Hind, L.E., Deng, Q., Beebe, D.J., Huttenlocher, A. (2017) Chemokine Signaling and the Regulation of Bidirectional Leukocyte Migration in Interstitial Tissues. Cell Reports. 19:1572-1585