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ZFIN ID: ZDB-LAB-170504-1
Kettunen Lab
PI/Director: Kettunen, Petronella
Contact Person: Kettunen, Petronella
Address: Department of Psychiatry and Neurochemistry Institute of Neuroscience and Physiology Sahlgrenska Academy University of Gothenburg Gothenburg Sweden
Line Designation: None assigned


Current research projects in the Kettunen lab include:
1. Molecular and cellular mechanisms of learning and memory
We are studying the molecular and cellular events that underlie learning. To this end, we are using the zebrafish (Danio rerio) and combining transgenic and molecular methods, behavioral experiments, in vivo calcium imaging and optogenetics.

We are also interested in human memory, memory testing and are associated with the Memory clinic in Mölndal, Sweden. Here, we are responsible for future genetic analysis of the patient material. In addition, Dr Kettunen is the co-director of the Center for Cognitive Medicine in Gothenburg, and organizes an annual PhD course in Learning and memory at University of Gothenburg.

2. Pathways underlying memory impairments and neurodegeneration in Alzheimer’s disease
We are interested in understanding how the Alzheimer’s disease-related peptide, amyloid-beta, is preventing memory formation in vivo. We are also studying the processes of amyloid-beta-mediated neurodegeneration and how these can be targeted for drug discovery. This project is supported by Sweden’s innovation agency VINNOVA.

3. The role of neuroinflammation in Alzheimer’s disease
In our preparation, we are able to follow the dynamics of microglia, i.e. the immune cells of the brain, and combine thse experiments with genetical analyses in human patient material. In this way, we hope to increase our understanding of the process of amyloid-beta clearance and the role of inflammation in Alzheimer’s disease. This project is supported by MORE (Mobility for Regional Excellence), Region VästraGötaland.

Within this project we collaborate with the labs of Morten Grøtli (University of Gothenburg) and Joakim Andréasson (Chalmers) to develop new photoprobes to manipulate inflammatory signalling in vivo.

4. Neuronal networks underlying social behavior
In collaboration with the Westberg lab at Sahlgrenska Academy, we are investigating the molecular pathways and transmitter systems underlying social behavior in mice and zebrafish. This project is supported by the Swedish Research Council for Medicine, ALF project funding and the Swedish Brain Foundation (Hjärnfonden).

5. Treatment strategies for brain tumors
In collaboration with the Carén lab at Sahlgrenska Cancer Center at University of Gothenburg, we use the zebrafish to investigate new treatments for brain tumors.

Landin, Jenny Post-Doc Näslund, Jakob Post-Doc Vindas, Marco A. Post-Doc

Midttun, H.L.E., Vindas, M.A., Whatmore, P.J., Øverli, Ø., Johansen, I.B. (2020) Effects of Pseudoloma neurophilia infection on the brain transcriptome in zebrafish (Danio rerio). Journal of fish diseases. 43(8):863-875
Landin, J., Hovey, D., Xu, B., Lagman, D., Zettergren, A., Larhammar, D., Kettunen, P., Westberg, L. (2020) Oxytocin Receptors Regulate Social Preference in Zebrafish. Scientific Reports. 10:5435
Kettunen, P. (2020) Calcium Imaging in the Zebrafish. Advances in experimental medicine and biology. 1131:901-942
Wenger, A., Larsson, S., Danielsson, A., Elbæk, K.J., Kettunen, P., Tisell, M., Sabel, M., Lannering, B., Nordborg, C., Schepke, E., Carén, H. (2017) Stem cell cultures derived from pediatric brain tumors accurately model the originating tumors. Oncotarget. 8(12):18626-18639
Banote, R.K., Edling, M., Eliassen, F., Kettunen, P., Zetterberg, H., Abramsson, A. (2016) β-Amyloid precursor protein-b is essential for Mauthner cell development in the zebrafish in a Notch-dependent manner. Developmental Biology. 413(1):26-38
Radomska, K.J., Sager, J., Farnsworth, B., Tellgren-Roth, Å., Tuveri, G., Peuckert, C., Kettunen, P., Jazin, E., Emilsson, L.S. (2016) Characterization and Expression of the Zebrafish qki Paralogs. PLoS One. 11:e0146155
Bliman, D., Nilsson, J.R., Kettunen, P., Andréasson, J., Grøtli, M. (2015) A Caged Ret Kinase Inhibitor and its Effect on Motoneuron Development in Zebrafish Embryos. Scientific Reports. 5:13109
Abramsson, A., Kettunen, P., Banote, R.K., Lott, E., Li, M., Arner, A., and Zetterberg, H. (2013) The zebrafish amyloid precursor protein-b is required for motor neuron guidance and synapse formation. Developmental Biology. 381(2):377-88
Kettunen, P. (2012) Calcium imaging in the zebrafish. Advances in experimental medicine and biology. 740
Issa, F.A., O'Brien, G., Kettunen, P., Sagasti, A., Glanzman, D.L., Papazian, D.M. (2011) Neural circuit activity in freely behaving zebrafish (Danio rerio). The Journal of experimental biology. 213(Pt 6):1028-1038
Abramsson, A., Westman-Brinkmalm, A., Pannee, J., Gustavsson, M., von Otter, M., Blennow, K., Brinkmalm, G., Kettunen, P., and Zetterberg, H. (2010) Proteomics Profiling of Single Organs from Individual Adult Zebrafish. Zebrafish. 7(2):161-168
Neveu, P., Sinha, D., Kettunen, P., Vriz, S., Julien, L., Bensimon, D. (2010) Single cell physiology. Proceedings of the Nobel Symposium on Single Molecule Spectroscopy in Chemistry, Physics and Biology. 96:305-316