ZFIN ID: ZDB-LAB-151106-1
Center for Medical Genetics Ghent (CMGG)
PI/Director: Willaert, Andy
Contact Person: Willaert, Andy
Email: Andy.Willaert@Ugent.be
URL: http://www.cmgg.be
Address: Ghent University Hospital Medical Research Building (MRB) De Pintelaan 185, B-9000 Ghent, Belgium
Country: Belgium
Phone: +32 9 332 4396
Fax: +32 9 332 6549
Line Designation: cmg

Show all 6 genomic features


Janssens, Els Post-Doc Coucke, Paul Speleman, Frank

Guillemyn, B., De Saffel, H., Bek, J.W., Tapaneeyaphan, P., De Clercq, A., Jarayseh, T., Debaenst, S., Willaert, A., De Rycke, R., Byers, P.H., Rosseel, T., Coucke, P., Blaumeiser, B., Syx, D., Malfait, F., Symoens, S. (2023) Syntaxin 18 Defects in Human and Zebrafish Unravel Key Roles in Early Cartilage and Bone Development. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 38(11):1718-1730
Daponte, V., Tonelli, F., Masiero, C., Syx, D., Exbrayat-Héritier, C., Biggiogera, M., Willaert, A., Rossi, A., Coucke, P.J., Ruggiero, F., Forlino, A. (2023) Cell differentiation and matrix organization are differentially affected during bone formation in osteogenesis imperfecta zebrafish models with different genetic defects impacting collagen type I structure. Matrix biology : journal of the International Society for Matrix Biology. 121:105-126
Jarayseh, T., Guillemyn, B., De Saffel, H., Bek, J.W., Syx, D., Symoens, S., Gansemans, Y., Van Nieuwerburgh, F., Jagadeesh, S., Raja, J., Malfait, F., Coucke, P.J., De Clercq, A., Willaert, A. (2023) A tapt1 knock-out zebrafish line with aberrant lens development and impaired vision models human early-onset cataract. Human genetics. 142(3):457-476
Tonelli, F., Leoni, L., Daponte, V., Gioia, R., Cotti, S., Fiedler, I.A.K., Larianova, D., Willaert, A., Coucke, P.J., Villani, S., Busse, B., Besio, R., Rossi, A., Witten, P.E., Forlino, A. (2023) Zebrafish Tric-b is required for skeletal development and bone cells differentiation. Frontiers in endocrinology. 14:10029141002914
Nunes, C., Depestel, L., Mus, L., Keller, K.M., Delhaye, L., Louwagie, A., Rishfi, M., Whale, A., Kara, N., Andrews, S.R., Dela Cruz, F., You, D., Siddiquee, A., Cologna, C.T., De Craemer, S., Dolman, E., Bartenhagen, C., De Vloed, F., Sanders, E., Eggermont, A., Bekaert, S.L., Van Loocke, W., Bek, J.W., Dewyn, G., Loontiens, S., Van Isterdael, G., Decaesteker, B., Tilleman, L., Van Nieuwerburgh, F., Vermeirssen, V., Van Neste, C., Ghesquiere, B., Goossens, S., Eyckerman, S., De Preter, K., Fischer, M., Houseley, J., Molenaar, J., De Wilde, B., Roberts, S.S., Durinck, K., Speleman, F. (2022) RRM2 enhances MYCN-driven neuroblastoma formation and acts as a synergistic target with CHK1 inhibition. Science advances. 8:eabn1382
Vierstraete, J., Fieuws, C., Creytens, D., Van Dorpe, J., Willaert, A., Vral, A., Claes, K.B. (2022) Atm deficient zebrafish model reveals conservation of the tumour suppressor function and a role in fertility. Genes & diseases. 10:381384381-384
Van Gils, M., Willaert, A., Coucke, P.J., Vanakker, O.M. (2022) The Abcc6a Knockout Zebrafish Model as a Novel Tool for Drug Screening for Pseudoxanthoma Elasticum. Frontiers in pharmacology. 13:822143
Nollet, L., Van Gils, M., Willaert, A., Coucke, P.J., Vanakker, O.M. (2021) Minocycline attenuates excessive DNA damage response and reduces ectopic calcification in pseudoxanthoma elasticum. The Journal of investigative dermatology. 142(6):1629-1638.e6
Pottie, L., Van Gool, W., Vanhooydonck, M., Hanisch, F.G., Goeminne, G., Rajkovic, A., Coucke, P., Sips, P., Callewaert, B. (2021) Loss of zebrafish atp6v1e1b, encoding a subunit of vacuolar ATPase, recapitulates human ARCL type 2C syndrome and identifies multiple pathobiological signatures. PLoS Genetics. 17:e1009603
Bek, J.W., Shochat, C., De Clercq, A., De Saffel, H., Boel, A., Metz, J., Rodenburg, F., Karasik, D., Willaert, A., Coucke, P.J. (2021) Lrp5 mutant and crispant zebrafish faithfully model human osteoporosis, establishing the zebrafish as a platform for CRISPR-based functional screening of osteoporosis candidate genes. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 36(9):1749-1764
Bek, J.W., De Clercq, A., Coucke, P.J., Willaert, A. (2021) The ZE-Tunnel: An Affordable, Easy-to-Assemble, and User-Friendly Benchtop Zebrafish Swim Tunnel. Zebrafish. 18(1):29-41
Delbaere, S., De Clercq, A., Mizumoto, S., Noborn, F., Bek, J.W., Alluyn, L., Gistelinck, C., Syx, D., Salmon, P.L., Coucke, P.J., Larson, G., Yamada, S., Willaert, A., Malfait, F. (2020) b3galt6 Knock-Out Zebrafish Recapitulate β3GalT6-Deficiency Disorders in Human and Reveal a Trisaccharide Proteoglycan Linkage Region. Frontiers in cell and developmental biology. 8:597857
Vierstraete, J., Fieuws, C., Willaert, A., Vral, A., Claes, K.B.M. (2020) Zebrafish as an in vivo screening tool to establish PARP inhibitor efficacy. DNA repair. 97:103023
Loontiens, S., Dolens, A.C., Strubbe, S., Van de Walle, I., Moore, F.E., Depestel, L., Vanhauwaert, S., Matthijssens, F., Langenau, D.M., Speleman, F., Van Vlierberghe, P., Durinck, K., Taghon, T. (2020) PHF6 Expression Levels Impact Human Hematopoietic Stem Cell Differentiation. Frontiers in cell and developmental biology. 8:599472
Naert, T., Tulkens, D., Edwards, N.A., Carron, M., Shaidani, N.I., Wlizla, M., Boel, A., Demuynck, S., Horb, M.E., Coucke, P., Willaert, A., Zorn, A.M., Vleminckx, K. (2020) Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos. Scientific Reports. 10:14662
Tonelli, F., Bek, J.W., Besio, R., De Clercq, A., Leoni, L., Salmon, P., Coucke, P.J., Willaert, A., Forlino, A. (2020) Zebrafish: A Resourceful Vertebrate Model to Investigate Skeletal Disorders. Frontiers in endocrinology. 11:489
Garcia, E.G., Veloso, A., Oliveira, M.L., Allen, J.R., Loontiens, S., Brunson, D., Do, D., Yan, C., Morris, R., Iyer, S., Garcia, S.P., Iftimia, N., Van Loocke, W., Matthijssens, F., McCarthy, K., Barata, J.T., Speleman, F., Taghon, T., Gutierrez, A., Van Vlierberghe, P., Haas, W., Blackburn, J.S., Langenau, D.M. (2020) PRL3 enhances T-cell acute lymphoblastic leukemia growth through suppressing T-cell signaling pathways and apoptosis. Leukemia. 35(3):679-690
Loontiens, S., Vanhauwaert, S., Depestel, L., Dewyn, G., Van Loocke, W., Moore, F.E., Garcia, E.G., Batchelor, L., Borga, C., Squiban, B., Malone-Perez, M., Volders, P.J., Olexiouk, V., Van Vlierberghe, P., Langenau, D.M., Frazer, J.K., Durinck, K., Speleman, F. (2020) A novel TLX1-driven T-ALL zebrafish model: comparative genomic analysis with other leukemia models. Leukemia. 34(12):3398-3403
Bek, J.W., De Clercq, A., De Saffel, H., Soenens, M., Huysseune, A., Witten, P.E., Coucke, P., Willaert, A. (2020) Photoconvertible fluorescent proteins: A versatile tool in zebrafish skeletal imaging. Journal of Fish Biology. 98(4):1007-1017
Watson, C.J., Monstad-Rios, A.T., Bhimani, R.M., Gistelinck, C., Willaert, A., Coucke, P., Hsu, Y.H., Kwon, R.Y. (2020) Phenomics-Based Quantification of CRISPR-Induced Mosaicism in Zebrafish. Cell systems. 10:275-286.e5
Delbaere, S., Van Damme, T., Syx, D., Symoens, S., Coucke, P., Willaert, A., Malfait, F. (2019) Hypomorphic zebrafish models mimic the musculoskeletal phenotype of β4GalT7-deficient Ehlers-Danlos syndrome. Matrix biology : journal of the International Society for Matrix Biology. 89:59-75
Oralová, V., Rosa, J.T., Soenens, M., Bek, J.W., Willaert, A., Witten, P.E., Huysseune, A. (2019) Beyond the whole-mount phenotype: high-resolution imaging in fluorescence-based applications on zebrafish. Biology Open. 8(5):
Loontiens, S., Depestel, L., Vanhauwaert, S., Dewyn, G., Gistelinck, C., Verboom, K., Van Loocke, W., Matthijssens, F., Willaert, A., Vandesompele, J., Speleman, F., Durinck, K. (2019) Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes. BMC Genomics. 20:228
Boel, A., De Saffel, H., Steyaert, W., Callewaert, B., De Paepe, A., Coucke, P.J., Willaert, A. (2018) CRISPR/Cas9-mediated homology-directed repair by ssODNs in zebrafish induces complex mutational patterns resulting from genomic integration of repair-template fragments. Disease models & mechanisms. 11(10)
Gistelinck, C., Kwon, R.Y., Malfait, F., Symoens, S., Harris, M.P., Henke, K., Hawkins, M.B., Fisher, S., Sips, P., Guillemyn, B., Bek, J.W., Vermassen, P., De Saffel, H., Witten, P.E., Weis, M., De Paepe, A., Eyre, D.R., Willaert, A., Coucke, P.J. (2018) Zebrafish type I collagen mutants faithfully recapitulate human type I collagenopathies. Proceedings of the National Academy of Sciences of the United States of America. 115(34):E8037-E8046
Garcia, E.G., Iyer, S., Garcia, S.P., Loontiens, S., Sadreyev, R.I., Speleman, F., Langenau, D.M. (2018) Cell of origin dictates aggression and stem cell number in acute lymphoblastic leukemia. Leukemia. 32:1860-1865
Van Gils, M., Willaert, A., De Vilder, E.Y.G., Coucke, P., Vanakker, O.M. (2018) Generation and Validation of a Complete Knockout Model of abcc6a In Zebrafish. The Journal of investigative dermatology. 138(11):2333-2342
Renard, M., Vanhauwaert, S., Vanhomwegen, M., Rihani, A., Vandamme, N., Goossens, S., Berx, G., Van Vlierberghe, P., Haigh, J.J., Decaesteker, B., Van Laere, J., Lambertz, I., Speleman, F., Vandesompele, J., Willaert, A. (2018) Expressed repetitive elements are broadly applicable reference targets for normalization of reverse transcription-qPCR data in mice. Scientific Reports. 8:7642
Vierstraete, J., Willaert, A., Vermassen, P., Coucke, P.J., Vral, A., Claes, K.B.M. (2017) Accurate quantification of homologous recombination in zebrafish: brca2 deficiency as a paradigm. Scientific Reports. 7:16518
Hur, M., Gistelinck, C.A., Huber, P., Lee, J., Thompson, M.H., Monstad-Rios, A.T., Watson, C.J., McMenamin, S.K., Willaert, A., Parichy, D.M., Coucke, P., Kwon, R.Y. (2017) MicroCT-Based Phenomics in the Zebrafish Skeleton Reveals Virtues of Deep Phenotyping in a Distributed Organ System. Zebrafish. 15(1):77-78
Hur, M., Gistelinck, C.A., Huber, P., Lee, J., Thompson, M.H., Monstad-Rios, A.T., Watson, C.J., McMenamin, S.K., Willaert, A., Parichy, D.M., Coucke, P., Kwon, R.Y. (2017) microCT-based phenomics in the zebrafish skeleton reveals virtues of deep phenotyping in a distributed organ system. eLIFE. 6
Gistelinck, C., Eckhard Witten, P., Huysseune, A., Symoens, S., Malfait, F., Larionova, D., Simoens, P., Dierick, M., Van Hoorebeke, L., De Paepe, A., Kwon, R.Y., Weis, M., Eyre, D.R., Willaert, A., Coucke, P.J. (2016) Loss of Type I Collagen Telopeptide Lysyl Hydroxylation Causes Musculoskeletal Abnormalities in a Zebrafish Model of Bruck Syndrome. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 31(11):1930-1942
Boel, A., Steyaert, W., De Rocker, N., Menten, B., Callewaert, B., De Paepe, A., Coucke, P., Willaert, A. (2016) BATCH-GE: Batch analysis of Next-Generation Sequencing data for genome editing assessment. Scientific Reports. 6:30330
Gistelinck, C., Gioia, R., Gagliardi, A., Tonelli, F., Marchese, L., Bianchi, L., Landi, C., Bini, L., Huysseune, A., Witten, P.E., Staes, A., Gevaert, K., De Rocker, N., Menten, B., Malfait, F., Leikin, S., Carra, S., Tenni, R., Rossi, A., De Paepe, A., Coucke, P., Willaert, A., Forlino, A. (2016) Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin. Scientific Reports. 6:21540
Vanhauwaert, S., Lefever, S., Coucke, P., Speleman, F., De Paepe, A., Vandesompele, J., Willaert, A. (2016) RT-qPCR gene expression analysis in zebrafish: Preanalytical precautions and use of expressed repetitive elements for normalization. Methods in cell biology. 135:329-42
Symoens, S., Barnes, A.M., Gistelinck, C., Malfait, F., Guillemyn, B., Steyaert, W., Syx, D., D'hondt, S., Biervliet, M., De Backer, J., Witten, E.P., Leikin, S., Makareeva, E., Gillessen-Kaesbach, G., Huysseune, A., Vleminckx, K., Willaert, A., De Paepe, A., Marini, J.C., Coucke, P.J. (2015) Genetic Defects in TAPT1 Disrupt Ciliogenesis and Cause a Complex Lethal Osteochondrodysplasia. American journal of human genetics. 97(4):521-34