ZFIN Lab: Stephens Lab

ZFIN ID: ZDB-LAB-140626-1

ZFIN ID: ZDB-LAB-140626-1

Stephens Lab

PI/Director:	Stephens, David
Contact Person:	Stephens, David
Email:	david.stephens@bristol.ac.uk
URL:	http://stephenslab.wordpress.com/
Address:	School of Biochemistry, University of Bristol, Medical Sciences Building, University Walk, BRISTOL, BS8 1TD
Country:	United Kingdom
Phone:	00441173312173
Fax:	00441173312168
Line Designation:	None assigned

GENOMIC FEATURES ORIGINATING FROM THIS LAB No data available

STATEMENT OF RESEARCH INTERESTS

Our work is directed at understanding the mechanisms that underlie membrane traffic, endomembrane organization and membrane-microtubule interactions in mammalian cells. The lab specializes in the analysis of membrane traffic in mammalian cells using advanced light microscopy techniques, in particular, the imaging of living cells. Our work includes developing imaging technologies and integrating model systems including 3D cultures and zebrafish to develop our research. Our main biological questions focus on the relationship between membrane trafficking and two important physiological processes: collagen secretion and cilia formation and function.

LAB MEMBERS

Miller, Vicky Post-Doc	Stevenson, Nicola Post-Doc	Bergen, Dylan Graduate Student
Evans, Ash Graduate Student

ZEBRAFISH PUBLICATIONS OF LAB MEMBERS

Bergen, D.J.M., Tong, Q., Shukla, A., Newham, E., Zethof, J., Lundberg, M., Ryan, R., Youlten, S.E., Frysz, M., Croucher, P.I., Flik, G., Richardson, R.J., Kemp, J.P., Hammond, C.L., Metz, J.R. (2022) Regenerating zebrafish scales express a subset of evolutionary conserved genes involved in human skeletal disease. BMC Biology. 20:21

Stevenson, N.L., Bergen, D.J.M., Lu, Y., Prada-Sanchez, M.E., Kadler, K.E., Hammond, C.L., Stephens, D.J. (2021) Giantin is required for intracellular N-terminal processing of type I procollagen. The Journal of cell biology. 220(6)

McDonald, G.L.K., Wang, M., Hammond, C.L., Bergen, D.J.M. (2021) Pharmacological Manipulation of Early Zebrafish Skeletal Development Shows an Important Role for Smad9 in Control of Skeletal Progenitor Populations. Biomolecules. 11(2):

Gregson, C.L., Bergen, D., Leo, P., Sessions, R.B., Wheeler, L., Hartley, A., Youlten, S., Croucher, P.I., McInerney-Leo, A.M., Fraser, W., Tang, J.C.Y., Anderson, L., Marshall, M., Sergot, L., Paternoster, L., Davey-Smith, G., AOGC Consortium, Brown, M.A., Hammond, C., Kemp, J.P., Tobias, J.H., Duncan, E.L. (2019) A rare mutation in SMAD9 associated with high bone mass identifies the SMAD-dependent BMP signalling pathway as a potential anabolic target for osteoporosis. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 35(1):92-105

Bergen, D.J.M., Kague, E., Hammond, C.L. (2019) Zebrafish as an Emerging Model for Osteoporosis: A Primary Testing Platform for Screening New Osteo-Active Compounds. Frontiers in endocrinology. 10:6

Stevenson, N.L., Bergen, D.J.M., Xu, A., Wyatt, E., Henry, F., McCaughey, J., Vuolo, L., Hammond, C.L., Stephens, D.J. (2018) Regulator of calcineurin-2 is a centriolar protein with a role in cilia length control. Journal of Cell Science. 131(9)

Stevenson, N.L., Bergen, D.J.M., Skinner, R.E.H., Kague, E., Martin-Silverstone, E., Robson Brown, K.A., Hammond, C.L., Stephens, D.J. (2017) Giantin knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression. Journal of Cell Science. 130(24):4132-4143

Bergen, D.J.M., Stevenson, N.L., Skinner, R.E.H., Stephens, D.J., Hammond, C.L. (2017) The Golgi matrix protein giantin is required for normal cilia function in zebrafish. Biology Open. 6(8):1180-1189

Guiu, J., Bergen, D.J., De Pater, E., Islam, A.B., Ayllón, V., Gama-Norton, L., Ruiz-Herguido, C., González, J., López-Bigas, N., Menendez, P., Dzierzak, E., Espinosa, L., Bigas, A. (2014) Identification of Cdca7 as a novel Notch transcriptional target involved in hematopoietic stem cell emergence. The Journal of experimental medicine. 211(12):2411-23

Williamson, R.C., Cowell, C.A., Hammond, C.L., Bergen, D., Roper, J., Feng, Y., Rendall, T.C., Race, P.R., Bass, M.D. (2014) Coronin-1C and RCC2 guide mesenchymal migration by trafficking Rac1 and controlling GEF exposure. Journal of Cell Science. 127(Pt 19):4292-307

Townley, A.K., Schmidt, K., Hodgson, L., and Stephens, D.J. (2012) Epithelial organization and cyst lumen expansion require efficient Sec13-Sec31-driven secretion. Journal of Cell Science. 125(3):673-684

Townley, A.K., Feng, Y., Schmidt, K., Carter, D.A., Porter, R., Verkade, P., and Stephens, D.J. (2008) Efficient coupling of Sec23-Sec24 to Sec13-Sec31 drives COPII-dependent collagen secretion and is essential for normal craniofacial development. Journal of Cell Science. 121(Pt 18):3025-3034