ZFIN ID: ZDB-LAB-010308-5
Gregg Lab
PI/Director: Gregg, Ronald G.
Contact Person: Gregg, Ronald G.
Email: ron.gregg@louisville.edu
URL: http://biochemistry.louisville.edu/faculty/faculty.php?id=4
Address: Depts. Biochemistry & Molecular Biology and Ophthalmology & Visual Sciences University of Louisville Baxter II, Room 221C 580 S. Preston Street Louisville, KY 40202 USA
Country: United States
Phone: (502) 852-4808
Fax: (502) 852-3659
Line Designation: None assigned


The laboratories focus is on structure and function of vertebrate retina. There three general research areas. The first involves studying the role of voltage gated calcium channels in retinal development. Mouse lines with various calcium channels inactivated by gene targeting are being studied. One of the knockout lines lacks neurotransmitter release in photoreceptor cells and this leads to a failure of normal synaptogenesis in the outer plexiform layer of the retina. This defect occurs early in development. Research focuses on gene expression during development in the mutant mice in an attempt to determine which genes are critical for normal synaptogenesis. The second area utilizes a mouse mutant , nob, the gene for which we have recetly cloned to begin to identify the mechanism by which glutamate interaction with the mGLUR6 on bipolar cells modulates the cation channel responsible for signaling in the bipolar cells. The third area of interest utilizes the advantages of using zebrafish as a system to study retinal development. The laboratory collaborates with Dr. John Dowling at Harvard University and Dr. Brian Link at the Medical College of Wisconsin. Dr. Link identified several zebrafish mutants that have defects in normal lamination in the retina. These genes are involved in critical stages of neural cell determination and differentiation and the Gregg lab is using positional cloning to identify the underlying genes.

Watson, Corey Research Staff Willer, Greg Research Staff

Gregg, R.K. (2021) Model Systems for the Study of Malignant Melanoma. Methods in molecular biology (Clifton, N.J.). 2265:1-21
Carmean, V., Yonkers, M.A., Tellez, M.B., Willer, J.R., Willer, G.B., Gregg, R.G., Geisler, R., Neuhauss, S.C., Ribera, A.B. (2015) pigk mutation underlies macho behavior and affects Rohon-Beard cell excitability. Journal of neurophysiology. 114(2):1146-57
Daly, C.M., Willer, J., Gregg, R., and Gross, J.M. (2013) snow white, a Zebrafish Model of Hermansky-Pudlak Syndrome Type 5. Genetics. 195(2):481-494
Peachey, N.S., Ray, T.A., Florijn, R., Rowe, L.B., Sjoerdsma, T., Contreras-Alcantara, S., Baba, K., Tosini, G., Pozdeyev, N., Iuvone, P.M., Bojang, P., Pearring, J.N., Simonsz, H.J., van Genderen, M., Birch, D.G., Traboulsi, E.I, Dorfman, A., Lopez, I., Ren, H., Goldberg, A.F., Nishina, P.M., Lachapelle, P., McCall, M.A., Koenekoop, R.K., Bergen, A.A., Kamermans, M., and Gregg, R.G. (2012) GPR179 Is Required for Depolarizing Bipolar Cell Function and Is Mutated in Autosomal-Recessive Complete Congenital Stationary Night Blindness. American journal of human genetics. 90(2):331-339
Tam, K.J., Watson, C.T., Massah, S., Kolybaba, A.M., Breden, F., Prefontaine, G.G., and Beischlag, T.V. (2011) Regulatory function of conserved sequences upstream of the long-wave sensitive opsin genes in teleost fishes. Vision Research. 51(21-22):2295-303
Murphy, T.R., Vihtelic, T.S., Ile, K.E., Watson, C.T., Willer, G.B., Gregg, R.G., Bankaitis, V.A., and Hyde, D.R. (2011) Phosphatidylinositol synthase is required for lens structural integrity and photoreceptor cell survival in the zebrafish eye. Experimental Eye Research. 93(4):460-74
Kur, E., Christa, A., Veth, K.N., Gajera, C.R., Andrade-Navarro, M.A., Zhang, J., Willer, J.R., Gregg, R.G., Abdelilah-Seyfried, S., Bachmann, S., Link, B.A., Hammes, A., and Willnow, T.E. (2011) Loss of Lrp2 in zebrafish disrupts pronephric tubular clearance but not forebrain development. Developmental dynamics : an official publication of the American Association of Anatomists. 240(6):1567-1577
Thomas, J.L., Vihtelic, T.S., Dendekker, A.D., Willer, G., Luo, X., Murphy, T.R., Gregg, R.G., Hyde, D.R., and Thummel, R. (2011) The loss of vacuolar protein sorting 11 (vps11) causes retinal pathogenesis in a vertebrate model of syndromic albinism. Investigative ophthalmology & visual science. 52(6):3119-28
Veth, K.N., Willer, J.R., Collery, R.F., Gray, M.P., Willer, G.B., Wagner, D.S., Mullins, M.C., Udvadia, A.J., Smith, R.S., John, S.W., Gregg, R.G., and Link, B.A. (2011) Mutations in Zebrafish lrp2 Result in Adult-Onset Ocular Pathogenesis That Models Myopia and Other Risk Factors for Glaucoma. PLoS Genetics. 7(2):e1001310
Haud, N., Kara, F., Diekmann, S., Henneke, M., Willer, J.R., Hillwig, M.S., Gregg, R.G., Macintosh, G.C., Gärtner, J., Alia, A., and Hurlstone, A.F. (2011) rnaset2 mutant zebrafish model familial cystic leukoencephalopathy and reveal a role for RNase T2 in degrading ribosomal RNA. Proceedings of the National Academy of Sciences of the United States of America. 108(3):1099-1103
Song, Y., Willer, J.R., Scherer, P.C., Panzer, J.A., Kugath, A., Skordalakes, E., Gregg, R.G., Willer, G.B., and Balice-Gordon, R.J. (2010) Neural and Synaptic Defects in slytherin, a Zebrafish Model for Human Congenital Disorders of Glycosylation. PLoS One. 5(10):e13743
Song, Y., Selak, M.A., Watson, C.T., Coutts, C., Scherer, P.C., Panzer, J.A., Gibbs, S., Scott, M.O., Willer, G., Gregg, R.G., Ali, D.W., Bennett, M.J., and Balice-Gordon, R.J. (2009) Mechanisms Underlying Metabolic and Neural Defects in Zebrafish and Human Multiple Acyl-CoA Dehydrogenase Deficiency (MADD). PLoS One. 4(12):e8329
Paulus, J.D., Willer, G.B., Willer, J.R., Gregg, R.G., and Halloran, M.C. (2009) Muscle contractions guide Rohon-Beard peripheral sensory axons. The Journal of neuroscience : the official journal of the Society for Neuroscience. 29(42):13190-13201
Lee, J., Willer, J.R., Willer, G.B., Smith, K., Gregg, R.G., and Gross, J.M. (2008) Zebrafish blowout provides genetic evidence for Patched1-mediated negative regulation of Hedgehog signaling within the proximal optic vesicle of the vertebrate eye. Developmental Biology. 319(1):10-22
Semina, E.V., Bosenko, D.V., Zinkevich, N.C., Soules, K.A., Hyde, D.R., Vihtelic, T.S., Willer, G.B., Gregg, R.G., and Link, B.A. (2006) Mutations in laminin alpha 1 result in complex, lens-independent ocular phenotypes in zebrafish. Developmental Biology. 299(1):63-77
Schroeter, E.H., Wong, R.O., and Gregg, R.G. (2006) In vivo development of retinal ON-bipolar cell axonal terminals visualized in nyx::MYFP transgenic zebrafish. Visual neuroscience. 23(5):833-843
Willer, G.B., Lee, V.M., Gregg, R.G., and Link, B.A. (2005) Analysis of the zebrafish perplexed mutation reveals tissue specific roles for de novo pyrimidine synthesis during development. Genetics. 170(4):1827-1837
Gregg, R.G., Willer, G.B., Fadool, J.M., Dowling, J.E., and Link, B.A. (2003) Positional cloning of the young mutation identifies an essential role for the Brahma chromatin remodeling complex in mediating retinal cell differentiation. Proceedings of the National Academy of Sciences of the United States of America. 100(11):6535-6540