MCM complex loading

Term ID

GO:0140530

Synonyms

• MCM complex loading at replication origin
• MCM double hexamer formation at replication origin

Definition

The protein localization process in which two MCM complexes become associated with chromatin at replication origins. MCM loading begins when origin-bound ORC and Cdc6 (Cdc18 in fission yeast) recruit one MCM2-7/Cdt1 complex to the origin, includes formation of a succession of intermediate complexes and ATP hydrolysis-dependent Mcm2-7 ring closure, and ends when two MCM hexamers fully encircle DNA, and are oriented head-to-head. The double hexamer is inactive for DNA unwinding. MCM loading takes place during G1 phase, and precedes CMG complex assembly. (4)

References

Ontology

GO: Biological Process    QuickGO    AmiGO