second spliceosomal transesterification activity

Term ID

GO:0000386

Synonyms

• 3'-splice site cleavage, exon ligation
• lariat formation, 5'-splice site cleavage

Definition

Catalysis of the second transesterification reaction of spliceosomal mRNA splicing. Ligation of the two exons occurs via a transesterification reaction where the free 3'-hydroxyl group of the 5' exon is the nucleophile attacking the 3' splice site. Non-expressed sequences are now detached from the exons. In cis splicing, the intron is in a lariat structure. (2)

References

• Reactome:R-HSA-9770236

Ontology

GO: Molecular Function    QuickGO    AmiGO