bacterial 2-hybrid assay evidence

Term ID

ECO:0007073

Synonyms

Definition

A type of bait-prey hybrid interaction evidence that involves the detection of protein-protein interaction by fusing the protein target (the "bait") to RNA polymerase and fusing protein or peptide library to be analyzed (the "prey") to DNA-binding domain. http://www.pnas.org/content/97/13/7382.full.pdf

References

Ontology

Evidence Ontology