bait-prey hybrid interaction evidence used in manual assertion

Term ID

ECO:0006077

Synonyms

bait-prey protein pull-down evidence

Definition

A type of bait-prey hybrid interaction evidence that is used in manual assertion where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein.

References

Ontology

Evidence Ontology

Relationships

is a type of: bait-prey hybrid interaction evidence bait-prey interaction evidence used in manual assertion

bait-prey hybrid interaction evidence bait-prey interaction evidence used in manual assertion Show first 5 Terms
has subtype: bacterial 2-hybrid assay evidence used in manual assertion bacterial one-hybrid evidence used in manual assertion glutathione S-transferase pull-down assay evidence used in manual assertion mammalian 2-hybrid assay evidence used in manual assertion yeast 2-hybrid evidence used in manual assertion ... Show All 6 Terms

bacterial 2-hybrid assay evidence used in manual assertion bacterial one-hybrid evidence used in manual assertion glutathione S-transferase pull-down assay evidence used in manual assertion mammalian 2-hybrid assay evidence used in manual assertion yeast 2-hybrid evidence used in manual assertion yeast one-hybrid evidence used in manual assertion Show first 5 Terms
used_in: manual assertion

manual assertion Show first 5 Terms