two-dimensional polyacrylamide gel electrophoresis evidence

Term ID

ECO:0005503

Synonyms

• 2D PAGE
• 2D-PAGE

Definition

A type of gel electrophoresis evidence that involves one dimension electrophoresis where biomolecules are electrophoresed on a low percentage polyacrylamide gel to be separated in proportion to their mass or isoelectric point, followed by high voltage electrophoresis on a higher percentage gel in the presence of ethidium bromide to alter non-linear molecular shape. http://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html

References

Ontology

Evidence Ontology