methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence

Term ID

ECO:0001814

Synonyms

• Methidiumpropyl-EDTA Fe(II) footprinting
• MPE Fe(II) footprinting
• MPE-EDTA Fe(II) footprinting

Definition

A type of nucleic acid binding evidence where the synthetic molecule methidiumpropyl-EDTA (MPE) is used to cleave ligand-protected DNA, followed by analysis of the restriction fragments to generate a footprint (i.e. size and location) of small molecule binding sites on the DNA. 6225070

References

Ontology

Evidence Ontology