polysome profiling evidence

Term ID

ECO:0001592

Synonyms

Definition

A type of experimental evidence employed to study the associations between multiple ribosomes and mRNA that utilizes density gradient centrifugation to separate out the lysate of cells of interest with the objective of isolating polysomes/polyribosomes which are complexes of an mRNA molecule and two or more ribosomes that act to translate mRNA. The optical density of the fractions is then determined. 30545912

References

Ontology

Evidence Ontology