chain termination sequencing evidence

Term ID

ECO:0000225

Synonyms

• dye terminator sequencing
• Sanger sequencing

Definition

A type of nucleotide sequencing assay in which DNA sequence is determined by addition of a primed DNA template to a reaction mixture, followed by addition of DNA polymerase, deoxynucleosidetriphosphates (dNTPs - one of which may be radiolabeled), and one of four di-deoxynucleotidetriphosphates (ddNTPs), after which DNA elongation is terminated and the DNA is separated by size and imaged.

References

Ontology

Evidence Ontology