electrophoretic mobility shift assay evidence

Term ID

ECO:0000096

Synonyms

• electrophoretic mobility shift assay
• EMSA: electrophoretic mobility shift assay
• Gel retardation assay

Definition

A type of affinity evidence based on an electrophoretic mobility shift of macromolecules, where proteins, nucleic acids, or both, are combined and the resulting mixtures are electrophoresed under native conditions through polyacrylamide or agarose gel to detect interactions/complexes between proteins and/or nucleic acid. 17703195

References

• MI:0413

Ontology

Evidence Ontology