far-Western blotting evidence

Term ID

ECO:0000076

Synonyms

• far-Western analysis

Definition

A type of physical interaction evidence that is based on detection of protein-protein interactions by separation of target proteins by SDS-PAGE which are blotted to a membrane, followed by denaturation and renaturation, probing with purified bait proteins, and detection of the target-bait complexes. 18079728

References

• MI:0047

Ontology

Evidence Ontology