differential methylation hybridization evidence

Term ID

ECO:0000069

Synonyms

• CpG island microarray evidence

Definition

A type of in vitro methylation assay evidence where methylation-sensitive restriction enzymes are utilized to compare differential methylation between two samples by first shearing DNA, followed by end-blunting, ligation of linkers, methylation sensitive restriction, PCR using linker primers, dye labeling and relative quantification of methylated DNA fragments by two-colored array hybridization to a CpG island microarray for visual assessment. 18987809

References

Ontology

Evidence Ontology