yeast 2-hybrid evidence

Term ID

ECO:0000068

Synonyms

• yeast two-hybrid assay

Definition

A type of bait-prey hybrid interaction evidence that is based on detection of protein-protein interaction by activation of a yeast reporter gene after a bait protein fused to a DNA-binding domain (which has been transfected into a yeast cell) is used to screen a cDNA library of clones fused to an activation domain. 12734586

References

Ontology

Evidence Ontology