Mapping Details

GENETIC MAPPING PANELS

Chr	Location	Mapped As	Panel	Mapped By	Scoring
2	38.3 cM	z9354	Boston MGH Cross (MGH)	Fishman, Mark C.	Data
2	1274.0 cR	z9354	Goodfellow T51 (T51)	Geisler, Robert	Data
Note: Physical map location as displayed in the genome browsers is more precise than linkage map location. Note: Physical map location should be used whenever possible.

MAPPING FROM PUBLICATIONS

Marker	Type	Chr	Distance	Publication / Person	Comments
z4300	SSLP	2		Fraenkel et al., 2009	Fraenkel et al (2009)localized tzhe067 to chromosome 2 using bulk segregant  ... Fraenkel et al (2009)localized tzhe067 to chromosome 2 using bulk segregant mapping. Intermediate resolution mapping with a panel of 88 mutant embryos identified flanking microsatellite markers z9354, z26250, and z4300.
z26250	SSLP	2		Fraenkel et al., 2009	Fraenkel et al (2009)localized tzhe067 to chromosome 2 using bulk segregant  ... Fraenkel et al (2009)localized tzhe067 to chromosome 2 using bulk segregant mapping. Intermediate resolution mapping with a panel of 88 mutant embryos identified flanking microsatellite markers z9354, z26250, and z4300.
tzhe067	Feature	2		Fraenkel et al., 2009	Fraenkel et al (2009)localized tzhe067 to chromosome 2 using bulk segregant  ... Fraenkel et al (2009)localized tzhe067 to chromosome 2 using bulk segregant mapping. Intermediate resolution mapping with a panel of 88 mutant embryos identified flanking microsatellite markers z9354, z26250, and z4300.

OTHER MAPPING INFORMATION

Chr 2 Fraenkel et al., 2009 Fraenkel et al (2009)localized tzhe067 to chromosome 2 using bulk segregant mapping. Intermediate  ... Fraenkel et al (2009)localized tzhe067 to chromosome 2 using bulk segregant mapping. Intermediate resolution mapping with a panel of 88 mutant embryos identified flanking microsatellite markers z9354, z26250, and z4300.

Primer Sets:

Strain	Bandsize	Restriction Enzyme	Annealing Temperature [C]
AB	275,0,231		60.0
Forward Primer	ATTGGTTTGCCCTTGAACAG
Reverse Primer	TGCCTCATCAGCTGTAAGCT
IND	0,219		60.0
Forward Primer	ATTGGTTTGCCCTTGAACAG
Reverse Primer	TGCCTCATCAGCTGTAAGCT
TU	251,0,231		60.0
Forward Primer	ATTGGTTTGCCCTTGAACAG
Reverse Primer	TGCCTCATCAGCTGTAAGCT
EKW	231,0,275		60.0
Forward Primer	ATTGGTTTGCCCTTGAACAG
Reverse Primer	TGCCTCATCAGCTGTAAGCT