Injection of two lats2 gRNAs leads to deletions and tumorigenesis. ( A) Schematic depicting the zebrafish lats2 gene and the PCR-based genotyping assay used to identify and differentiate between successful large deletion alleles and WT alleles. Arrows indicate the target sites of the gRNAs used. F refers to a forward primer; R refers to a reverse primer. ( B) Agarose gel separation of PCR amplicons generated based on the genotyping assay depicted in ( A). Amplicons of the correct size, indicative of a large genomic deletion, are present in lats2 CRISPR-injected embryos, but absent in uninjected negative controls. Numbers above lanes represent the number of embryos used as the template DNA for each PCR sample. First lane contains a 100-bp ladder (NEB). ( C– H’) Six examples of lats2 CRISPR-injected fish that developed tumors by 6 mpf. Arrows denote tumors when not obviously visible. ( I) Kaplan‒Meier plot of tumor-free survival in lats2 CRISPR-injected fish.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.