| ZFIN ID: ZDB-EXP-261002-13 |
Experiment Conditions Description: primary cell culture: regenerating fin, chemical treatment by environment: ionomycin
| primary cell culture: regenerating fin |
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| Name: | primary cell culture |
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| Synonyms: | |
| Definition: | In vitro culture condition in which an embryo or adult fish is dissociated to a single cell suspension. The analysis is made on this cell culture. |
| Ontology: | Zebrafish Environment Condition Ontology [ZECO:0000145] |
| Name: | regenerating fin |
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| Synonyms: | regenerating fins |
| Definition: | Fin undergoing the process of regeneration. |
| Ontology: | Anatomy Ontology [ZFA:0001269] |
| chemical treatment by environment: ionomycin |
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| Name: | chemical treatment by environment |
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| Synonyms: | |
| Definition: | Chemical treatment condition in which the chemical is introduced through the environment. For zebrafish this is the tank water. |
| Ontology: | Zebrafish Environment Condition Ontology [ZECO:0000238] |
| Name: | ionomycin |
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| Synonyms: | (4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-11,19,21-trihydroxy-22-{(2S,2'R,5S,5'S)-5'-[(1R)-1-hydroxyethyl]-2,5'-dimethyloctahydro-2,2'-bifuran-5-yl}-4,6,8,12,14,18,20-heptamethyl-9-oxodocosa-10,16-dienoic acid |
| Definition: | A very long-chain fatty acid that is docosa-10,16-dienoic acid which is substituted by methyl groups at positions 4, 6, 8, 12, 14, 18 and 20, by hydroxy groups at positions 11, 19 and 21, and by a (2',5-dimethyloctahydro-2,2'-bifuran-5-yl)ethanol group at position 21. An ionophore produced by Streptomyces conglobatus, it is used in research to raise the intracellular level of Ca2+ and as a research tool to understand Ca2+ transport across biological membranes. |
| Ontology: | ChEBI [CHEBI:63954] ( EBI ) |
| Publication: | Le Bleu et al., 2025 |
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