Transgenic Construct
Tg(hsp70l:wnt9b-IRES-EGFP)
- ID
- ZDB-TGCONSTRCT-221027-2
- Name
- Tg(hsp70l:wnt9b-IRES-EGFP)
- Previous Names
- None
- Type
- engineered_plasmid
- Regulatory Regions
- Coding Sequences
- Contains Sequences
- None
- Note
-
The open reading frames of zebrafish wnt9b (ENSDARG00000037889) was amplified by PCR with AK3 and AK4 primers (Table S1) and cloned into the Gateway pDONR221 vector by Gateway BP cloning (Thermo Fisher Scientific) to generate pME-Wnt9b. pDest_Tol2pA_hsp70l:wnt9b_IRES_EGFP was created by Gateway LR cloning (Thermo Fisher Scientific) from pDest_Tol2pA, p5E-hsp70l, pME-Wnt9b and p3E-IRES-eGFPpA. 15pg of plasmid was injected into 1-cell stage embryos with 15pg of Tol2 transposase mRNA.
Plasmid Map
None
Genomic Features
That Utilize tg(hsp70l:wnt9b-ires-egfp) Construct
| Genomic Feature | Affected Genomic Regions |
|---|---|
| pbb48Tg |
Transgenics
That Utilize tg(hsp70l:wnt9b-ires-egfp) Construct
| Fish | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|
| pbb48Tg/pbb48Tg + MO2-wnt9b + MO3-wnt9a |
|
Fig. 5.
from
Paolini et al., 2023
|
|
| pbb48Tg/pbb48Tg; twu34Tg/twu34Tg |
|
Fig. 2.
from
Paolini et al., 2023
|
Sequence Information
Other Construct Pages
None
Citations