Transgenic Construct
Tg(T2OncZ,actb1:RFP)
- ID
- ZDB-TGCONSTRCT-110127-24
- Name
- Tg(T2OncZ,actb1:RFP)
- Previous Names
- Type
- engineered_plasmid
- Regulatory Regions
- Coding Sequences
- Contains Sequences
- None
- Note
-
A modified carp Beta-actin promoter was cloned into the Sleeping Beauty transposon, T2/Onc, behind the murine stem cell virus 5' LTR to create T2/OncZ (T2OncZ). To create transgenic lines, linear fragments of T2/OncZ were mixed and injected with linear fragments of a Beta-actin:RFP reporter cassette.
Plasmid Map
None
Genomic Features
That Utilize Tg(T2OncZ,actb1:RFP) Construct
Transgenics
That Utilize Tg(T2OncZ,actb1:RFP) Construct
| Fish | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|
| is6Tg |
|
||
| is6Tg/+ (AB) |
|
||
| is6Tg/+ (WIK) |
|
||
| is7Tg |
|
||
| is7Tg (AB) |
|
3 figures
from
Calvird et al., 2022
|
4 figures
from
Calvird et al., 2022
|
| is7Tg + MO1-nsd1a (AB) |
|
FIGURE 4
from
Calvird et al., 2022
|
|
| is7Tg + MO1-nsd1a + MO1-nsd1b (AB) |
|
FIGURE 4
from
Calvird et al., 2022
|
|
| is7Tg + MO1-nsd1b (AB) |
|
FIGURE 4
from
Calvird et al., 2022
|
|
| is7Tg + MO2-cbx5 (AB) |
|
FIGURE 3
from
Calvird et al., 2022
|
|
| is7Tg + MO2-kmt5c (AB) |
|
FIGURE 3
from
Calvird et al., 2022
|
|
| is7Tg + MO2-suv39h1b (AB) |
|
Fig. S2 from Calvird et al., 2022 |
FIGURE 2
from
Calvird et al., 2022
|
| is7Tg + MO2-zbtb24 (AB) |
|
FIGURE 3
from
Calvird et al., 2022
|
|
| is7Tg + MO3-ehmt2 (AB) |
|
FIGURE 2
from
Calvird et al., 2022
|
|
| is7Tg + MO3-setdb2 (AB) |
|
FIGURE 2
from
Calvird et al., 2022
|
Sequence Information
Other Construct Pages
None
Citations