PUBLICATION
Tdr2, a new zebrafish transposon of the Tc1 family
- Authors
- Göttgens, B., Barton, L.M., Grafham, D., Vaudin, M., and Green, A.R.
- ID
- ZDB-PUB-991112-3
- Date
- 1999
- Source
- Gene 239(2): 373-379 (Journal)
- Registered Authors
- Green, Andrea
- Keywords
- cruciform; inverted; transposase; repeat
- MeSH Terms
-
- Sequence Analysis, DNA
- Animals
- Transposases/genetics
- Molecular Sequence Data
- DNA Transposable Elements/genetics*
- Sequence Alignment
- Amino Acid Sequence
- Sequence Homology, Amino Acid
- Repetitive Sequences, Nucleic Acid/genetics
- Zebrafish/genetics*
- Sequence Homology, Nucleic Acid
- DNA/chemistry
- DNA/genetics
- Base Sequence
- Phylogeny
- PubMed
- 10548740 Full text @ Gene
Citation
Göttgens, B., Barton, L.M., Grafham, D., Vaudin, M., and Green, A.R. (1999) Tdr2, a new zebrafish transposon of the Tc1 family. Gene. 239(2):373-379.
Abstract
We describe here Tdr2, a new class of Tc1-like transposons in zebrafish. Tdr2 was identified from the genomic sequence of a zebrafish PAC (P1 artificial chromosome) clone, and fragments of Tdr2 were found in several zebrafish EST (expressed sequence tag) sequences. Predicted translation of the Tdr2 transposase gene showed that it was most closely related to Caenorhabditis elegans Tc3A, suggesting an ancient origin of the Tdr2 transposon. Tdr2 spans 1.1kb and is flanked by inverted repeats of approx. 100bp. The 5' repeat is itself composed of an inverted repeat, raising the possibility of the formation of a cruciform DNA structure. Tdr2 transposons may facilitate the development of novel transposon-based tools for the genetic analysis of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping