PUBLICATION
Predictability of dorso-ventral asymmetry in the cleavage stage zebrafish embryo: an analysis using lithium sensitivity as a dorso-ventral marker
- Authors
- Aanstad, P. and Whitaker, M.
- ID
- ZDB-PUB-991102-21
- Date
- 1999
- Source
- Mechanisms of Development 88(1): 33-41 (Journal)
- Registered Authors
- Aanstad, Pia
- Keywords
- zebrafish; dorso-ventral axis specification; lithium
- MeSH Terms
-
- Blastomeres
- Animals
- Guanosine 5'-O-(3-Thiotriphosphate)/metabolism
- Microinjections
- Zebrafish/embryology*
- Embryonic Induction
- Biomarkers
- Lithium/pharmacology*
- Fluorescent Dyes/metabolism
- Organic Chemicals
- Embryo, Nonmammalian/drug effects
- Body Patterning/physiology*
- PubMed
- 10525186 Full text @ Mech. Dev.
Citation
Aanstad, P. and Whitaker, M. (1999) Predictability of dorso-ventral asymmetry in the cleavage stage zebrafish embryo: an analysis using lithium sensitivity as a dorso-ventral marker. Mechanisms of Development. 88(1):33-41.
Abstract
The dorso-ventral axis in zebrafish first becomes apparent at gastrulation, when the future ventral side appears thinner than the dorsal side. The exact time of
establishment of the dorso-ventral axis is not known. We show here that the dorso-ventral axis is specified as early as the 32 cell stage. Using lithium as a marker for dorso-ventral asymmetry, we show that lithium-sensitivity is a characteristic of future ventral cell, but not future dorsal cells, and that there is an asymmetric lithium-sensitivity along the long axis of the 32 cell stage embryo. Consequently, the dorso-ventral axis corresponds to the long axis of the embryo. Because the effect of lithium treatment is short-lived, the dorso-ventral axis must be specified in zebrafish already at the 32 cell stage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping