PUBLICATION
Zebrafish semaphorin Z1b inhibits growing motor axons in vivo
- Authors
- Roos, M., Schachner, M., and Bernhardt, R.R.
- ID
- ZDB-PUB-991020-5
- Date
- 1999
- Source
- Mechanisms of Development 87(1-2): 103-117 (Journal)
- Registered Authors
- Bernhardt, Robert, Roos, Marc, Schachner, Melitta
- Keywords
- embryo; somites; rhombomeres; recognition molecules; collapsin; semaphorin; axon guidance; repulsion; motor axon; overexpression
- MeSH Terms
-
- Motor Neurons/cytology
- Motor Neurons/metabolism
- Molecular Sequence Data
- In Situ Hybridization
- DNA, Complementary/metabolism
- Animals
- Axons/physiology
- Cloning, Molecular
- Blotting, Western
- Amino Acid Sequence
- Zebrafish
- Nerve Growth Factors/genetics
- Nerve Growth Factors/metabolism*
- Nerve Growth Factors/physiology*
- Time Factors
- Somites/metabolism
- Zebrafish Proteins
- Protein Biosynthesis
- Mutagenesis
- Gene Expression Regulation, Developmental*
- RNA, Messenger/metabolism
- Sequence Homology, Amino Acid
- PubMed
- 10495275 Full text @ Mech. Dev.
Citation
Roos, M., Schachner, M., and Bernhardt, R.R. (1999) Zebrafish semaphorin Z1b inhibits growing motor axons in vivo. Mechanisms of Development. 87(1-2):103-117.
Abstract
Zebrafish semaphorin 1b (sema Z1b) is a new member of the semaphorin family, related to mammalian sema D/III. It is expressed in rhombomeres three and five, and in the posterior half of newly formed somites which is avoided by ventrally extending motor axons. Embryos injected at the 1-2 cell stage with synthetic sema Z1b mRNA developed normally but many (63%) showed missing or severely stunted ventral motor nerves. Other axons, somites, and hindbrain rhombomeres were not affected. No abnormalities were seen in control embryos injected with lacZ mRNA. Sema Z1b might normally influence the midsegmental pathway choice of the ventrally extending motor axons by contributing to a repulsive domain in the posterior somite.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping