PUBLICATION
Promoter activity of the zebrafish bhikhari retroelement requires an intact activin signaling pathway
- Authors
- Vogel, A.M. and Gerster, T.
- ID
- ZDB-PUB-990824-26
- Date
- 1999
- Source
- Mechanisms of Development 85(1-2): 133-146 (Journal)
- Registered Authors
- Gerster, Thomas, Vogel, Andreas M.
- Keywords
- mesoderm induction; bhikhari; activin signaling pathway; retrotransposon; fibroblast growth factor
- MeSH Terms
-
- Activins
- Animals
- Embryonic Induction/genetics
- Gene Expression Regulation, Developmental*
- Inhibins/genetics*
- Mesoderm
- Promoter Regions, Genetic*
- Retroelements/genetics*
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 10415354 Full text @ Mech. Dev.
Citation
Vogel, A.M. and Gerster, T. (1999) Promoter activity of the zebrafish bhikhari retroelement requires an intact activin signaling pathway. Mechanisms of Development. 85(1-2):133-146.
Abstract
We have investigated mesoderm induction in zebrafish employing the zebrafish LTR-retroelement bhikhari (bik). bik elements are transcribed in all early mesendodermal cells. This expression pattern is generated by a promoter located in the U3 region of the LTR. We show that bik is activated through the activin/Vg1 signaling pathway in an immediate early fashion. This activation critically depends on a sequence motif that occurs among others also in the Xenopus Mix2 activin response element (ARE). It has been shown that the Mix2 ARE binds FAST- 1, which complexes with Smad proteins to form a multi-protein complex. We confirm that also the bik ARE can be bound by FAST-1 in vitro. In animal cap experiments we demonstrate that this binding site is required for activin-induced transcriptional activation mediated by FAST and Smad-type proteins.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping