PUBLICATION
Hox gene complexity in medaka fish may be similar to that in pufferfish rather than zebrafish
- Authors
- Kurosawa, G., Yamada, K., Ishiguro, H., and Hori, H.
- ID
- ZDB-PUB-990628-9
- Date
- 1999
- Source
- Biochemical and Biophysical Research Communications 260(1): 66-70 (Journal)
- Registered Authors
- Hori, Hiroshi
- Keywords
- Hox genes; medaka fish; Oryzias latipes
- MeSH Terms
-
- Animals
- Base Sequence
- Evolution, Molecular
- Fishes/genetics*
- Homeodomain Proteins/genetics*
- Molecular Sequence Data
- Multigene Family
- Oryzias/genetics*
- Sequence Homology, Amino Acid
- Sequence Homology, Nucleic Acid
- Zebrafish/genetics*
- PubMed
- 10381345 Full text @ Biochem. Biophys. Res. Commun.
Citation
Kurosawa, G., Yamada, K., Ishiguro, H., and Hori, H. (1999) Hox gene complexity in medaka fish may be similar to that in pufferfish rather than zebrafish. Biochemical and Biophysical Research Communications. 260(1):66-70.
Abstract
Changes in number and the genomic organization of Hox genes have played an important role in metazoan body-plan evolution. They make cluster(s), and in vertebrates, each cluster contains different number of Hox genes that have been classified into 13 groups. There are 39 Hox genes in four clusters on different chromosomes in the mammalian genome. In the fish, while 31 Hox genes in four clusters have been identified in pufferfish Fugu rubripes, 47 Hox genes in seven clusters exist in the zebrafish Danio rerio. To estimate the evolutionary origin of Hox organization in ray-finned fishes, we searched for Hox genes in the medaka fish Oryzias latipes, with a taxon thought to be widely separated from those of pufferfish and zebrafish. We synthesized various mixed oligonucleotides that can work as group-specific primers for PCR, then cloned and sequenced amplified fragments. Numbers of Hox genes identified in the present study were 2 for group 1, 2 for group 2, 1 for group 3, 3 for group 4, 6 for groups 5-7, 2 for group 8, 4 for group 9, 3 for group 10, 1 for group 12, and 3 for group 13. The primers specific for group 11 did not function in this study. Thus, at least 27 Hox genes are present in medaka genome, suggesting that the Hox gene complexity of the medaka genome is similar to that of the pufferfish rather than the zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping