ZFIN ID: ZDB-PUB-990628-18
Maturation of neuromuscular transmission during early development in zebrafish
Nguyen, P.V., Aniksztejn, L., Catarsi, S., and Drapeau, P.
Date: 1999
Source: Journal of neurophysiology   81(6): 2852-2861 (Journal)
Registered Authors: Aniksztejn, Laurent, Drapeau, Pierre, Nguyen, Peter V.
Keywords: none
MeSH Terms:
  • Animals
  • Cholinesterase Inhibitors/pharmacology
  • Electric Stimulation
  • Embryo, Nonmammalian
  • In Vitro Techniques
  • Kinetics
  • Larva
  • Motor Endplate/growth & development
  • Motor Endplate/physiology
  • Muscle Fibers, Skeletal/physiology
  • Neuromuscular Junction/growth & development
  • Neuromuscular Junction/physiology*
  • Patch-Clamp Techniques
  • Physostigmine/pharmacology
  • Receptors, Cholinergic/metabolism
  • Synaptic Transmission/physiology*
  • Zebrafish
PubMed: 10368402
Maturation of neuromuscular transmission during early development in zebrafish. We have examined the rapid development of synaptic transmission at the neuromuscular junction (NMJ) in zebrafish embryos and larvae by patch-clamp recording of spontaneous miniature endplate currents (mEPCs) and single acetylcholine receptor (AChR) channels. Embryonic (24-36 h) mEPCs recorded in vivo were small in amplitude (<50 pA). The rate of mEPCs increased in larvae (3.5-fold increase measured by 6 days), and these mEPCs were mostly of larger amplitude (10-fold on average) with (