|ZFIN ID: ZDB-PUB-990628-18|
Maturation of neuromuscular transmission during early development in zebrafish
Nguyen, P.V., Aniksztejn, L., Catarsi, S., and Drapeau, P.
|Source:||Journal of neurophysiology 81(6): 2852-2861 (Journal)|
|Registered Authors:||Aniksztejn, Laurent, Drapeau, Pierre, Nguyen, Peter V.|
Nguyen, P.V., Aniksztejn, L., Catarsi, S., and Drapeau, P. (1999) Maturation of neuromuscular transmission during early development in zebrafish. Journal of neurophysiology. 81(6):2852-2861.
ABSTRACTMaturation of neuromuscular transmission during early development in zebrafish. We have examined the rapid development of synaptic transmission at the neuromuscular junction (NMJ) in zebrafish embryos and larvae by patch-clamp recording of spontaneous miniature endplate currents (mEPCs) and single acetylcholine receptor (AChR) channels. Embryonic (24-36 h) mEPCs recorded in vivo were small in amplitude (<50 pA). The rate of mEPCs increased in larvae (3.5-fold increase measured by 6 days), and these mEPCs were mostly of larger amplitude (10-fold on average) with (=5-fold) faster kinetics. Intracellular labeling with Lucifer yellow indicated extensive coupling between muscle cells in both embryos and larvae (=10 days). Blocking acetylcholinesterase (AChE) with eserine had no effect on mEPC kinetics in embryos at 1 day and only partially slowed (by approximately 1/2) the decay rate in larvae at 6 days. In acutely dissociated muscle cells, we observed the same two types of AChR with conductances of 45 and 60 pS and with similar, brief (<0.5 ms) mean open times in both embryos and larvae. We conclude that AChR properties are set early during development at these early stages; functional maturation of the NMJ is only partly shaped by expression of AChE and may also depend on postsynaptic AChR clustering and presynaptic maturation.
ADDITIONAL INFORMATION No data available