ZFIN ID: ZDB-PUB-990616-2
In vivo recording from identifiable neurons of the locomotor network in the developing zebrafish
Drapeau, P., Ali, D.W., Buss, R.R., and Saint-Amant, L.
Date: 1999
Source: Journal of Neuroscience Methods   88(1): 1-13 (Review)
Registered Authors: Buss, Robert, Drapeau, Pierre, Saint-Amant, Louis
Keywords: patch clamp; hindbrain; spinal cord; swimming; embryo; rhythmic activity
MeSH Terms:
  • Action Potentials/drug effects
  • Action Potentials/physiology
  • Animals
  • Bicuculline/pharmacology
  • Embryo, Nonmammalian/physiology
  • Excitatory Amino Acid Antagonists/pharmacology
  • GABA Antagonists/pharmacology
  • Glycine/physiology
  • Kynurenic Acid/pharmacology
  • Motor Neurons/physiology*
  • Patch-Clamp Techniques
  • Periodicity
  • Rhodamines
  • Rhombencephalon/cytology
  • Rhombencephalon/embryology
  • Rhombencephalon/physiology
  • Spinal Cord/cytology
  • Spinal Cord/embryology
  • Spinal Cord/physiology
  • Swimming/physiology*
  • Tetrodotoxin/pharmacology
  • Zebrafish/embryology*
PubMed: 10379574 Full text @ J. Neurosci. Methods
ABSTRACT
The zebrafish is a popular model for developmental studies due to its accessibility by cellular, molecular and genetic approaches. As a complement to these other methods, we have devised an exposed hindbrain/spinal cord preparation in the curarized zebrafish embryo and larva that permits intracellular labeling and patch clamp recording from individually identified sensory neurons, motoneurons and interneurons in vivo. Regular bursts of synaptic potentials and action potentials were observed under whole-cell current clamp in embryonic motoneurons and in some identified interneurons. Larval neurons showed prolonged depolarizations with synaptically driven bursts of action potentials. Frequent spontaneous synaptic potentials were observed and synaptic currents were effectively space clamped. It is thus feasible to study in vivo the properties of identifiable neurons of the developing locomotor network in the zebrafish, including their synaptic activity, firing patterns and interconnections.
ADDITIONAL INFORMATION No data available