PUBLICATION
The zebrafish detour gene is essential for cranial but not spinal motor neuron induction
- Authors
- Chandrasekhar, A., Schauerte, H.E., Haffter, P., and Kuwada, J.Y.
- ID
- ZDB-PUB-990525-4
- Date
- 1999
- Source
- Development (Cambridge, England) 126(12): 2727-2737 (Journal)
- Registered Authors
- Chandrasekhar, Anand, Haffter, Pascal, Kuwada, John, Schauerte, Heike
- Keywords
- zebrafish; hindbrain; rhombomere; cranial motor neuron; spinal cord; detour; sonic hedgehog; protein kinase A; hedgehog signaling
- MeSH Terms
-
- Transcription Factors*
- Tumor Suppressor Proteins
- Proteins/genetics
- Proteins/metabolism
- Zebrafish/embryology
- Zebrafish/genetics*
- Animals
- Embryo, Nonmammalian
- Mutation*
- Embryonic Induction/genetics
- Trans-Activators*
- Contactin 2
- Rhombencephalon/embryology*
- Rhombencephalon/metabolism
- Hedgehog Proteins
- Motor Neurons/physiology*
- Cyclic AMP-Dependent Protein Kinases/genetics
- Cyclic AMP-Dependent Protein Kinases/metabolism
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- Cell Adhesion Molecules, Neuronal/genetics
- Cell Adhesion Molecules, Neuronal/metabolism
- Spine/embryology
- Spine/innervation*
- Signal Transduction
- Gene Expression Regulation, Developmental
- Basic Helix-Loop-Helix Transcription Factors
- Nerve Growth Factors/genetics
- Nerve Growth Factors/metabolism
- Zebrafish Proteins*
- PubMed
- 10331983 Full text @ Development
Citation
Chandrasekhar, A., Schauerte, H.E., Haffter, P., and Kuwada, J.Y. (1999) The zebrafish detour gene is essential for cranial but not spinal motor neuron induction. Development (Cambridge, England). 126(12):2727-2737.
Abstract
The zebrafish detour (dtr) mutation generates a novel neuronal phenotype. In dtr mutants, most cranial motor neurons, especially the branchiomotor, are missing. However, spinal motor neurons are generated normally. The loss of cranial motor neurons is not due to aberrant hindbrain patterning, failure of neurogenesis, increased cell death or absence of hh expression. Furthermore, activation of the Hh pathway, which normally induces branchiomotor neurons, fails to induce motor neurons in the dtr hindbrain. Despite this, not all Hh-mediated regulation of hindbrain development is abolished since the regulation of a neural gene by Hh is intact in the dtr hindbrain. Finally, dtr can function cell autonomously to induce branchiomotor neurons. These results suggest that detour encodes a component of the Hh signaling pathway that is essential for the induction of motor neurons in the hindbrain but not in the spinal cord and that dtr function is required for the induction of only a subset of Hh-mediated events in the hindbrain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping