PUBLICATION
A new secreted protein that binds to Wnt proteins and inhibits their activities
- Authors
- Hsieh, J.C., Kodjabachian, L., Rebbert, M.L., Rattner, A., Smallwood, P.M., Samos, C.H., Nusse, R., Dawid, I.B., and Nathans, J.
- ID
- ZDB-PUB-990507-11
- Date
- 1999
- Source
- Nature 398(6726): 431-436 (Journal)
- Registered Authors
- Dawid, Igor B., Kodjabachian, Laurent
- Keywords
- none
- MeSH Terms
-
- Zebrafish
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Wnt1 Protein
- Intracellular Signaling Peptides and Proteins
- Proto-Oncogene Proteins/antagonists & inhibitors
- Proto-Oncogene Proteins/metabolism*
- Mice
- Sequence Homology, Amino Acid
- Zebrafish Proteins*
- Amino Acid Sequence
- Animals
- Xenopus
- Humans
- Protein Binding
- CHO Cells
- Cricetinae
- Extracellular Matrix Proteins
- Carrier Proteins/genetics
- Carrier Proteins/isolation & purification
- Carrier Proteins/metabolism*
- Intercellular Signaling Peptides and Proteins
- Adaptor Proteins, Signal Transducing
- Signal Transduction
- Wnt Proteins
- Molecular Sequence Data
- Xenopus Proteins
- Repressor Proteins*
- Drosophila Proteins*
- Immunoglobulin G/genetics
- Immunoglobulin G/metabolism
- PubMed
- 10201374 Full text @ Nature
Citation
Hsieh, J.C., Kodjabachian, L., Rebbert, M.L., Rattner, A., Smallwood, P.M., Samos, C.H., Nusse, R., Dawid, I.B., and Nathans, J. (1999) A new secreted protein that binds to Wnt proteins and inhibits their activities. Nature. 398(6726):431-436.
Abstract
The Wnt proteins constitute a large family of extracellular signalling molecules that are found throughout the animal kingdom and are important for a wide variety of normal and pathological developmental processes. Here we describe Wnt-inhibitory factor-1 (WIF-1), a secreted protein that binds to Wnt proteins and inhibits their activities. WIF-1 is present in fish, amphibia and mammals, and is expressed during Xenopus and zebrafish development in a complex pattern that includes paraxial presomitic mesoderm, notochord, branchial arches and neural crest derivatives. We use Xenopus embryos to show that WIF-1 overexpression affects somitogenesis (the generation of trunk mesoderm segments), in agreement with its normal expression in paraxial mesoderm. In vitro, WIF-1 binds to Drosophila Wingless and Xenopus Wnt8 produced by Drosophila S2 cells. Together with earlier results obtained with the secreted Frizzled-related proteins, our results indicate that Wnt proteins interact with structurally diverse extracellular inhibitors, presumably to fine-tune the spatial and temporal patterns of Wnt activity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping