PUBLICATION
Region-specific cell clones in the developing spinal cord of the zebrafish
- Authors
- Papan, C. and Campos-Ortega, J.A.
- ID
- ZDB-PUB-990427-18
- Date
- 1999
- Source
- Development genes and evolution 209(3): 135-144 (Journal)
- Registered Authors
- Campos-Ortega, Jose, Papan, Cyrus
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Count
- Cell Division
- Clone Cells/cytology
- Dextrans/administration & dosage
- Fluoresceins/administration & dosage
- Microinjections
- Spinal Cord/cytology*
- Spinal Cord/embryology*
- Zebrafish/embryology*
- PubMed
- 10079356 Full text @ Dev. Genes Evol.
Citation
Papan, C. and Campos-Ortega, J.A. (1999) Region-specific cell clones in the developing spinal cord of the zebrafish. Development genes and evolution. 209(3):135-144.
Abstract
To analyse the proliferative abilities of cells within particular regions of the zebrafish neural plate, injections of fluorescein-dextran were made into single cells at either medial or intermediary positions in the neural plate region of two-somite stage embryos. The resulting cell clones were analysed in 3. 5-day-old embryos. Clones with similar compositions were found among those derived from injections in both regions, and these were grouped into classes. 78 clones 29 obtained following injections in the medial region, and 22 of 59 cell clones derived from injections in the intermediary region, were classifiable into 9 and 10 different classes, respectively, each comprising a variable number of clones. Several identified cell types, as well as each of the clone classes themselves, were specific for the regions of the neural plate from which they derived, i.e. they were not represented among the clones derived from the other region. These results suggest that the composition of the lineages derived from particular cells is constant in different animals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping