PUBLICATION

An activated form of type I serine/threonine kinase receptor TARAM-A reveals a specific signalling pathway involved in fish head organiser formation

Authors

Renucci, A., Lemarchandel, V., and Rosa, F.

ID

ZDB-PUB-990416-1

Date

1996

Source

Development (Cambridge, England) 122: 3735-3743 (Journal)

Registered Authors

Lemarchandel, Valerie, Renucci, Armand, Rosa, Frederic

Keywords

none

MeSH Terms

Receptors, Transforming Growth Factor beta*
Body Patterning/genetics*
Zebrafish/embryology
Sequence Analysis, DNA
Amino Acid Sequence
Mesoderm/physiology
Head/embryology
Protein Serine-Threonine Kinases/genetics
Protein Serine-Threonine Kinases/metabolism*
Animals
Embryonic Induction/genetics*
Tissue Distribution
Sequence Homology, Amino Acid
Cloning, Molecular
Molecular Sequence Data
Receptors, Growth Factor/genetics
Receptors, Growth Factor/metabolism*
DNA, Complementary/genetics

(all 18)

PubMed

9012495 Full text @ Development

Abstract

The role of Transforming Growth Factor beta (TGF-beta)-related molecules in axis formation and mesoderm patterning in vertebrates has been extensively documented, but the identity and mechanisms of action of the endogenous molecules remained uncertain. In this study, we isolate a novel serine/threonine kinase type I receptor, TARAM-A, expressed during early zebrafish embryogenesis first ubiquitously and then restricted to dorsal mesoderm during gastrulation. A constitutive form of the receptor is able to induce the most anterior dorsal mesoderm rapidly and to confer an anterior organizing activity. By contrast, the wild-type form is only able to induce a local expansion of the dorsal mesoderm. Thus an activated form of TARAM-A is sufficient to induce dorsoanterior structures and TARAM-A may be activated by dorsally localized signals. Our data suggest the existence in fish of a specific TGF-beta-related pathway for anterior dorsal mesoderm induction, possibly mediated by TARAM-A and activated at the late blastula stage by localized dorsal determinant.

Genes / Markers

Figures

Show all Figures

Expression

Fish	Conditions	Stage	Anatomy	Assay	Figure
WT	standard conditions	Bud	axial hypoblast	ISH	Fig. 2
WT	standard conditions	90%-epiboly	axial hypoblast	ISH	Fig. 2
WT	standard conditions	256-cell	blastodisc	ISH	Fig. 2
WT	standard conditions	2-cell	blastodisc	ISH	Fig. 2
WT	standard conditions	Shield	DEL	ISH	Fig. 2
WT	standard conditions	90%-epiboly	epiblast	ISH	Fig. 2
WT	standard conditions	75%-epiboly	hypoblast	ISH	Fig. 2
WT	standard conditions	30%-epiboly	margin	ISH	Fig. 2
WT	standard conditions	Shield	margin	ISH	Fig. 2
WT	standard conditions	Dome	marginal blastomere	ISH	Fig. 2

1 - 10 of 12

Show

Phenotype

Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
WT

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-ta	polyclonal	tbxta		Rabbit

Orthology

Engineered Foreign Genes

Mapping

Renucci et al., 1996 ZDB-PUB-990416-1 PMID:9012495

An activated form of type I serine/threonine kinase receptor TARAM-A reveals a specific signalling pathway involved in fish head organiser formation

Renucci et al., 1996

ZDB-PUB-990416-1
PMID:9012495