PUBLICATION

The effects of temperature on the dark-adapted spectral sensitivity function of the adult zebrafish

Authors
Saszik, S. and Bilotta, J.
ID
ZDB-PUB-990405-9
Date
1999
Source
Vision Research   39: 1051-1058 (Journal)
Registered Authors
Bilotta, Joe
Keywords
Danio rerio; dark-adapted spectral sensitivity; electroretinogram; temperature
MeSH Terms
  • Adaptation, Ocular/physiology*
  • Animals
  • Electroretinography
  • Female
  • Male
  • Photic Stimulation
  • Retinal Cone Photoreceptor Cells/physiology
  • Retinal Pigments/physiology*
  • Retinal Rod Photoreceptor Cells/physiology
  • Rhodopsin/physiology
  • Temperature*
  • Zebrafish/physiology*
PubMed
10343824 Full text @ Vision Res.
Abstract
In goldfish and other cold-blooded vertebrates, temperature can influence the rhodopsin/porphyropsin contributions to the rod photoreceptors. This study examined the effects of temperature on the spectral sensitivity function of the dark-adapted zebrafish. Zebrafish were housed in either a warm (28-30 degrees C) or cold (22-25 degrees C) tank prior to testing. Fish were dark-adapted for at least 1 h and electroretinogram (ERG) responses to 200 ms stimuli of various wavelengths and irradiances were obtained. Results show that water temperature affected the spectral sensitivity function of the ERG b-wave. Subjects housed in the warm temperatures had a spectral sensitivity consistent with the rhodopsin absorption curve; however, fish housed in the colder temperatures had a spectral sensitivity function that was the result of a rhodopsin/porphyropsin mixture. In addition, ultraviolet cones (lambda max: 362 nm) contributed to the dark-adapted spectral sensitivity function under both temperature conditions. Consistent with the results from other fish, the dark-adapted visual system of the zebrafish can be influenced by water temperature. The results of this study demonstrate the necessity of maintaining a stable environment when examining the contributions of the photoreceptors to the visual response.
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Errata and Notes