Isolation of zebrafish gdf7 and comparative genetic mapping of genes belonging to the growth/differentiation factor 5, 6, 7 subgroup of the TGF-beta superfamily
- Davidson, A.J., Postlethwait, J.H., Yan, Y.-L., Beier, D.R., Van Doren, C., Förnzler, D., Celeste, A.J., Crosier, K.E., and Crosier, P.S.
- Genome research 9: 121-129 (Journal)
- Registered Authors
- Beier, David R., Crosier, Kathy, Crosier, Phil, Davidson, Alan, Postlethwait, John H., Yan, Yi-Lin
- MeSH Terms
- Amino Acid Sequence
- Base Sequence
- Bone Morphogenetic Proteins*
- Chromosome Mapping
- DNA/isolation & purification
- Gene Expression Regulation
- Growth Differentiation Factor 5
- Growth Differentiation Factor 6
- Growth Differentiation Factors
- Growth Substances/genetics*
- Growth Substances/isolation & purification*
- Molecular Sequence Data
- Multigene Family/genetics*
- Sequence Homology, Amino Acid
- Transforming Growth Factor beta/genetics*
- Zebrafish Proteins
- 10022976 Full text @ Genome Res.
Davidson, A.J., Postlethwait, J.H., Yan, Y.-L., Beier, D.R., Van Doren, C., Förnzler, D., Celeste, A.J., Crosier, K.E., and Crosier, P.S. (1999) Isolation of zebrafish gdf7 and comparative genetic mapping of genes belonging to the growth/differentiation factor 5, 6, 7 subgroup of the TGF-beta superfamily. Genome research. 9:121-129.
The Growth/differentiation factor (Gdf) 5, 6, 7 genes form a closely related subgroup belonging to the TGF-beta superfamily. In zebrafish, there are three genes that belong to the Gdf5, 6, 7 subgroup that have been named radar, dynamo, and contact. The genes radar and dynamo both encode proteins most similar to mouse GDF6. The orthologous identity of these genes on the basis of amino acid similarities has not been clear. We have identified gdf7, a fourth zebrafish gene belonging to the Gdf5, 6, 7 subgroup. To assign correct orthologies and to investigate the evolutionary relationships of the human, mouse, and zebrafish Gdf5, 6, 7 subgroup, we have compared genetic map positions of the zebrafish and mammalian genes. We have mapped zebrafish gdf7 to linkage group (LG) 17, contact to LG9, GDF6 to human chromosome (Hsa) 8 and GDF7 to Hsa2p. The radar and dynamo genes have been localized previously to LG16 and LG19, respectively. A comparison of syntenies shared among human, mouse, and zebrafish genomes indicates that gdf7 is the ortholog of mammalian GDF7/Gdf7. LG16 shares syntenic relationships with mouse chromosome (Mmu) 4, including Gdf6. Portions of LG16 and LG19 appear to be duplicate chromosomes, thus suggesting that radar and dynamo are both orthologs of Gdf6. Finally, the mapping data is consistent with contact being the zebrafish ortholog of mammalian GDF5/Gdf5.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes