PUBLICATION
Promoter structure of human sonic hedgehog gene
- Authors
- Kitazawa, S., Kitazawa, R., Tamada, H., and Maeda, S.
- ID
- ZDB-PUB-990316-2
- Date
- 1998
- Source
- BBA Gene Structure and Expression 1443: 358-363 (Journal)
- Registered Authors
- Keywords
- Base Sequence; Binding Sites; Bucladesine; Cholecalciferol; Dexamethasone; DNA; Estradiol; Gene Expression Regulation, Neoplastic; Genes, Structural; Human; Molecular Sequence Data; Promoter Regions (Genetics); Proteins; Sequence Analysis, DNA; Sequence Deletion; Support, Non-U.S. Gov't; Tetradecanoylphorbol Acetate; Tretinoin; Tumor Cells, Cultured; TATA Box
- MeSH Terms
-
- Tumor Cells, Cultured/cytology
- Tumor Cells, Cultured/drug effects
- Tumor Cells, Cultured/metabolism
- Tetradecanoylphorbol Acetate/pharmacology
- Trans-Activators*
- Bucladesine/pharmacology
- Estradiol/pharmacology
- DNA/chemistry
- DNA/genetics
- Humans
- Promoter Regions, Genetic/genetics*
- TATA Box
- Base Sequence
- Molecular Sequence Data
- Hedgehog Proteins
- Cholecalciferol/pharmacology
- Dexamethasone/pharmacology
- Sequence Analysis, DNA
- Gene Expression Regulation, Neoplastic
- Binding Sites
- Sequence Deletion
- Genes/genetics*
- Proteins/genetics*
- Tretinoin/pharmacology
- PubMed
- 9878825 Full text @ BBA Gene Structure and Expression
Citation
Kitazawa, S., Kitazawa, R., Tamada, H., and Maeda, S. (1998) Promoter structure of human sonic hedgehog gene. BBA Gene Structure and Expression. 1443:358-363.
Abstract
Sonic hedgehog (Shh) is a secreted signal transducer responsible not only for patterning of the anterior-posterior axis during early limb and neuronal development, but also for generating cell-type diversity at later developmental stages. To elucidate the mechanism regulating human Shh gene expression, we cloned the 5'-flanking region of the human Shh gene and characterized it by transient transfection studies. Two transcription start sites were identified by primer extension analysis. Two TATA-boxes, a CCAAT-box and a palindrome-like structure constituted the basic promoter structure. Furthermore, two continuous E-boxes and a putative homeodomain containing an ATTA-box were located around 350-450 bp upstream of the upper TATA-box. Consensus binding sites of the RA, estrogen, D3 and glucocorticoid/progesterone receptors were not found within the cloned sequence. Short-term treatment with TPA increased luciferase activity up to 2.1-fold; on the other hand, treatment with dibutyryl-cyclic AMP decreased it to 0.8-fold. Retinoic acid (RA), vitamin D3, dexamethazone (DEX) and estradiol (E2) had no effect on the luciferase activity. Since the zebrafish Shh promoter contains two closely spaced axial (HNF3beta) binding sequences on its basic promoter, the palindrome-like structure located in the corresponding site of the human Shh promoter may be a crucial binding domain regulating human Shh gene expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping